- Clone
- 3C7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3C7_PEDZL_CD25_Antibody_121417 3C7_PEDZL_CD25_Antibody_121417](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3C7_PEDZL_CD25_Antibody_121417.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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ConA-stimulated BALB/c splenocytes (Day 3) stained with CD25 (Clone 3C7) PE/Dazzle™ 594 (filled histogram) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
101919 | 25 µg | ¥25,960 | |
101920 | 100 µg | ¥68,420 |
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-2-dependent BALB/c mouse helper T-cell clone HT-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
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Application References
(PubMed link indicates BioLegend citation) -
- Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- RRID
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AB_2721701 (BioLegend Cat. No. 101919)
AB_2721702 (BioLegend Cat. No. 101920)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
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Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor, signal transduction
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. - Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
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