- Clone
- 2A9-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- V28, GPR13, Chemokine C-X3-C receptor 1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2A9-1_PerCPCyanine55_CXCR1_Antibody_1_030424 2A9-1_PerCPCyanine55_CXCR1_Antibody_1_030424](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2A9-1_PerCPCyanine55_CXCR1_Antibody_1_030424.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD3 FITC and CX3CR1 (clone 2A9-1) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Save |
---|---|---|---|
341613 | 25 tests | ¥36,300 | |
341614 | 100 tests | ¥84,040 |
CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CX3CR1-EGFP fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Additional Product Notes
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BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Nishimura M, et al. 2002. J. Immunol. 168:6173.
- Nanki T, et al. 2002. Arthritis Rheum. 46:2878.
- Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837.
- Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
- Product Citations
- RRID
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AB_11218599 (BioLegend Cat. No. 341613)
AB_11219203 (BioLegend Cat. No. 341614)
Antigen Details
- Structure
- G-protein-coupled seven transmembrane receptor
- Distribution
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NK cells, T cell subset, monocytes/macrophages, dendritic cells
- Function
- cell adhesion and migration
- Ligand/Receptor
- CX3CL1 (Fractalkine, FKN, neurotactin)
- Cell Type
- Dendritic cells, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Cytokine/Chemokine Receptors, GPCR
- Antigen References
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1. Imai T, et al. 1997. Cell. 91:521.
2. Fong AM, et al. 1998. J. Exp. Med. 188:1413.
3. Auffray C, et al. 2009. J. Exp. Med. 206:595. - Gene ID
- 1524 View all products for this Gene ID
- UniProt
- View information about CX3CR1 on UniProt.org
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
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Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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