- Clone
- L17F12 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- II T7
- Other Names
- Leu-1, Ly-1, T1, Tp67, Leu1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_L17F12_Purified_CD5_Antibody_FC_102714 1_L17F12_Purified_CD5_Antibody_FC_102714](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L17F12_Purified_CD5_Antibody_FC_102714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified CD5 (clone L17F12, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC. -
Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-human CD20 (clone 2H7) Alexa Fluor® 488 (green) and 10 µg/ml of purified anti-human CD5 (clone L17F12) overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG2a (clone RMG2a-62) Alexa Fluor® 594 (red) for two hours at room temperature. The image was captured by 10X objective.
Cat # | Size | Price | Save |
---|---|---|---|
364001 | 25 µg | ¥11,880 | |
364002 | 100 µg | ¥22,220 |
CD5, also known as Leu-1, Ly-1 and T1, is a 67 kD single chain type I glycoprotein that is a member of the scavenger receptor superfamily. CD5 is expressed on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions. One of its ligands is CD72.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human T-acute lymphoblastic leukemia (ALL) cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats): immunoprecipitation1,3,4 and immmunohistochemical staining of frozen and formalin-fixed paraffin-embedded sections2,3,5.
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Application References
(PubMed link indicates BioLegend citation) -
- McAlister MS, et al. 1998. Protein Eng. 11:847. (IP)
- Butmarc JR, et al. 1998. Am. J. Clin. Pathol. 109:682. (IHC)
- Engleman EG, et al. 1981. Proc. Natl. Acad. Sci. USA 78:1791. (IHC, IP)
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310. (FC, IP)
- Warnke R and Levy R. 1980. J. Histochem. Cytochem. 28:771. (IHC)
- Product Citations
- RRID
-
AB_2564476 (BioLegend Cat. No. 364001)
AB_2564477 (BioLegend Cat. No. 364002)
Antigen Details
- Structure
- Scavenger receptor superfamily, 67 kD
- Distribution
-
Thymocytes, T cells, and subsets of B and dendritic cells.
- Function
- Modulates TCR and BCR signaling, involved in thymocyte maturation and T-B cell interaction
- Ligand/Receptor
- CD72
- Cell Type
- B cells, Dendritic cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kipps T. 1989. Adv. Immunol. 47:117.
2. Resnick D, et al. 1994. Trends Biochem. Sci. 1:5.
3. Wood GS and Freudenthal PS. 1992. Am. J. Pathol. 141:789. - Gene ID
- 921 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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