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Purified anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_Purified_HLA-DR_Antibody_012519_updated.png
Human peripheral blood lymphocytes were stained with CD19 APC and HLA-DR (clone L243) Purified (left) or Purified mouse IgG2a, κ isotype control (right) followed by anti-mouse IgG FITC.
  • L243_Purified_HLA-DR_Antibody_012519_updated.png
    Human peripheral blood lymphocytes were stained with CD19 APC and HLA-DR (clone L243) Purified (left) or Purified mouse IgG2a, κ isotype control (right) followed by anti-mouse IgG FITC.
Cat # Size Price Save
307602 100 µg ¥15,840
Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IP, WB, Block, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  22. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  2. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  3. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  4. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  5. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  6. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  7. Olin A, et al. 2018. Cell. 174:1277. PubMed
  8. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  9. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  10. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  11. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  12. Gravastrand CS, et al. 2019. J Immunol. 203(4). PubMed
  13. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  14. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
  15. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  16. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  17. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  18. Lu Y, et al. 2017. Cell Mol Immunol. 1.054166667. PubMed
  19. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  20. Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed
  21. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  22. Mirandola L, et al. 2017. Oncotarget. 8:74378. PubMed
  23. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  24. Wu Y, et al. 2018. J Immunol. 200:1627. PubMed
  25. Aljabri A, et al. 2019. Cell Death Dis. 0.579861111. PubMed
  26. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  27. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  28. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  29. Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
  30. Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
  31. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  32. Tsuruta M, et al. 2018. Oncoimmunology. 7:e1415687. PubMed
  33. Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed
  34. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  35. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  36. Zeiner PS, et al. 2018. Acta Neuropathol Commun. 6:18. PubMed
  37. Scholzen A, et al. 2019. Front Immunol. 0.560416667. PubMed
  38. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  39. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  40. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  41. Nagamatsu T, et al. 2009. Hum Reprod. 24:3160. PubMed
  42. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  43. Salvat R, et al. 2014. J Vis Exp. 85: 51308. PubMed
  44. Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
  45. Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed
  46. Tal MC, et al. 2020. MBio. 11:00. PubMed
  47. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  48. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  49. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  50. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  51. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  52. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  53. Henrick BM, et al. 2021. Cell. . PubMed
  54. Schwabenland M, et al. 2021. Immunity. . PubMed
  55. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  56. Parker R, et al. 2020. bioRxiv. . PubMed
  57. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  58. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  59. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  60. Raffray L, et al. 2020. Immunology. 159:88. PubMed
  61. Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
  62. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  63. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
  64. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  65. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  66. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  67. Macmillan ML, et al. 2021. Blood Adv. 5:1425. PubMed
  68. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  69. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  70. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  71. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  72. Liu R, et al. 2022. Curr Protoc. 2:e350. PubMed
  73. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  74. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  75. Law JC, et al. 2021. J Immunol. 206:37. PubMed
  76. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  77. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  78. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  79. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
RRID
AB_314680 (BioLegend Cat. No. 307602)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

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