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- Clone
- JES1-39D10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Save |
|---|---|---|---|
| 500902 | 500 µg | ¥57,640 |
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS-expressed, recombinant human IL-5
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IHC-F, ICC, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-5 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA1-3 or ELISPOT4-6 Capture: The purified JES1-39D10 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the biotinylated JES1-5A10 (Cat. No. 501002) antibody as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500907).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 & 430404) are specially developed and recommended. -
Application References
(PubMed link indicates BioLegend citation) -
- Abrams, J., et al. 1992. Immunol. Rev. 127:5.
- Butterfield, J., et al. 1992. Blood 79:688.
- Abrams, J., et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker, New York. p.133.
- Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.6.
- Mahanty, S., et al. 1992. J. Immunol. 148:3567.
- Limaye, A., et al. 1991. J. Clin. Inv. 88:1418.
- Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
- Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Andersson, J., et al. 1994. Immunology 83:16.
- Product Citations
- RRID
-
AB_315137 (BioLegend Cat. No. 500902)
Antigen Details
- Structure
- Cytokine; dimer; 45 kD (Mammalian)
- Bioactivity
- Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
- Cell Sources
- Mast cells, T cells, bone marrow stromal cells
- Cell Targets
- Eosinophils
- Receptors
- Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immun. 35:593. - Regulation
- Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
- Gene ID
- 3567 View all products for this Gene ID
- UniProt
- View information about IL-5 on UniProt.org
Related Pages & Pathways
Pathways
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