- Clone
- S15049I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- M130, MM130, SCARI1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S15049I_Pure_CD163_Antibody_1_043018 S15049I_Pure_CD163_Antibody_1_043018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S15049I_Pure_CD163_Antibody_1_043018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse peritoneal macrophages were cultured for seven days in the presence of Dexamethasone, then stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody. -
C57BL/6 bone marrow were stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody. Data shown was gated on total bone marrow cells. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD163 Antibody (clone S15049I) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) and CD3e (clone 500A2) Alexa Fluor® 647 (green) for two hours at room temperature. The image was captured with a 10X objective. -
C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD163 Antibody (clone S15049I) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
Cat # | Size | Price | Save |
---|---|---|---|
155302 | 500 µg | ¥64,460 |
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD163 extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
- RRID
-
AB_2734239 (BioLegend Cat. No. 155302)
Antigen Details
- Structure
- Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
- Distribution
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Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells
- Function
- Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- 93671 View all products for this Gene ID
- UniProt
- View information about CD163 on UniProt.org
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