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Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_Pure_060308
TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
  • G8dot8_Pure_060308
    TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
Cat # Size Price Save
118201 50 µg ¥12,640
118202 500 µg ¥50,200
Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  2. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  3. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  4. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  5. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
  6. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  7. Iyer N, et al. 2020. PLoS Pathog. 16:e1008360. PubMed
  8. Nudel I, et al. 2011. J Immunol. 186:891. PubMed
  9. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  10. Ishii K, et al. 2012. Development. 139:1734. PubMed
  11. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  12. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  13. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  14. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  15. Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
  16. Huang L, et al. 2020. JCI Insight. 5:00. PubMed
  17. Foster DS, et al. 2020. Nat Commun. 3.278472222. PubMed
  18. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  19. Molina LM, et al. 2021. Cell Reports. 36(1):109310. PubMed
  20. Corcos N, et al. 2021. Frontiers in Immunology. 12:616215. PubMed
  21. E-Lacerda RR, et al. 2020. Clin Exp Allergy. 256:50. PubMed
  22. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  23. Thorsen AS, et al. 2020. Dis Model Mech. . PubMed
  24. Zhou Y, et al. 2020. Am J Pathol. 190:1414. PubMed
  25. Billings SE, et al. 2021. Development. 148: . PubMed
  26. Fuhrmann L, et al. 2021. Int J Mol Sci. 22:. PubMed
  27. Thorsen AS, et al. 2020. Dis Model Mech. 14:dmm046706. PubMed
  28. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  29. Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
  30. Van Winkle JA, et al. 2020. J Virol. 94:. PubMed
  31. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  32. Yamamoto K, et al. 2022. STAR Protoc. 3:101155. PubMed
  33. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  34. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  35. Tomchaney M, et al. 2021. BMC Pulm Med. 21:275. PubMed
  36. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  37. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  38. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  39. Karabacak NM, et al. 2022. Cancer Res. 82:1084. PubMed
  40. Tremblay M, et al. 2020. Elife. 9: . PubMed
  41. Nozaki K, et al. 2022. Nature. 606:960. PubMed
RRID
AB_1089026 (BioLegend Cat. No. 118201)
AB_1089027 (BioLegend Cat. No. 118202)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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