- Clone
- Gal397 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Gal397_a_Purified_Galectin-3_Mac-2_Antibody_WB_081516 Gal397_a_Purified_Galectin-3_Mac-2_Antibody_WB_081516](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Gal397_a_Purified_Galectin-3_Mac-2_Antibody_WB_081516.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Galectin-3 (Mac-2) antibody (clone Gal397). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. -
Total cell lysates (15 µg protein) from NIH3T3 (lane 1), HT29 (lane 2) and 293E (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-mouse/human Galectin-3 (Mac-2) antibody (clone Gal397) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-mouse/human Galectin-3 (Mac-2) antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder. -
HeLa cells were stained with purified anti-Galectin 3 (Gal397) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).
Cat # | Size | Price | Save |
---|---|---|---|
126701 | 50 µg | ¥15,620 | |
126702 | 500 µg | ¥56,760 |
Galectin-3 (galactose-specific lectin 3) is a glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.
Product DetailsProduct Details
- Reactivity
- Mouse,Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Recombinant (partial), amino acids 151-251
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of < 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Based on immunogen information, clone Gal397 may recognize African Green Baboon and Cynomolgus. It is recommended to test the reagent for optimal performance for other species. Due to in-house testing, the purified format is not recommended for flow cytometry and intracellular flow cytometry.
- Product Citations
- RRID
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AB_1134255 (BioLegend Cat. No. 126701)
AB_1134256 (BioLegend Cat. No. 126702)
Antigen Details
- Structure
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Human Galetcin-3 has 250 amino acids with a predicted molecular weight of 26 kD.
Mouse Galetcin-3 has 264 amino acids with a predicted molecular weight of 27.5 kD. - Distribution
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Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells
- Function
- Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
- Ligand/Receptor
- Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
- Cell Type
- Dendritic cells, Epithelial cells, Macrophages, Monocytes
- Biology Area
- Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules
- Antigen References
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- Kim HRC, et al. 1999. Cancer Res. 59:4148
- Reljic R, et al. 2004. Immunol. Lett. 93:51.
- Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395
- Joo GH, et al. 2001. J. Leukoc. Biol. 69:555
- Llinas L, et al. 2011. Immunol. Lett. 134:113-21
- Cabezón R, et al. 2011. Immunol. Lett. 134:167-73
- Gene ID
- 16854 View all products for this Gene ID 3958 View all products for this Gene ID
- UniProt
- View information about Mac-2 on UniProt.org
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