- Clone
- A15158C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 1, Transcription factor ISGF-3 components p91/p84
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15158C_Pure_Stat1_Antibody_1_082918 A15158C_Pure_Stat1_Antibody_1_082918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15158C_Pure_Stat1_Antibody_1_082918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from U3A (negative control, Lane 1), Jurkat (Lane 2), and HeLa cells treated without (Lane 3) or with (Lane 4) 500 ng/mL nocodazole overnight were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-STAT1 clone A15158C (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301) for clone A15158C, and HRP donkey anti-rabbit IgG (Cat. No. 406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: MW ladder. -
To confirm that the described nocodazole treatment worked, total cell lysates (15 µg total protein) from HeLa cells treated without (-) or with (+) nocodazole 500 ng/mL overnight were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) purified anti-STAT1 Serine 727 antibody (clone A15158B, left) or purified anti-STAT1 antibody (clone A15158C, right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301). -
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.05 µg/mL (1:10000 dilution) of Purified anti-STAT1 Antibody, clone A15158C, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # | Size | Price | Save |
---|---|---|---|
603701 | 25 µg | ¥22,220 | |
603702 | 100 µg | ¥55,660 |
STAT1, also known as signal transducter and activator of transcription 1, is a ubiquitously expressed protein that is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, cytosolic STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcriptional activator. Two isoforms of STAT1, with predicted molecular weights of 87 (STAT1α) and 83 kD (STAT1β), exist as a result of alternative RNA processing. This clone is specific for STAT1α. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human STAT1 peptide phosphorylated at Serine 727.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.25 µg per ml (1:2000 - 1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is specific for STAT1 isoform a and is not predicted to recognize the ß isoform due to the absence of the region corresponding to the immunizing peptide.
This clone displayed a stronger affinity for STAT1a in parallel western blot testing with BioLegend’s anti-STAT1 antibody (clone 10C4B40).
U3A cells were selected for antibody specificity testing because the cell line does not express STAT11.
This clone was derived from a human STAT1 peptide phosphorylated at Serine 727 immunogen, but tested negative for phospho-recognition while displaying strong pan-reactivity. Supplemental data is provided confirming the differences in phosphorylation of STAT1 Serine 727 in HeLa cells treated with or without nocodazole. -
Application References
(PubMed link indicates BioLegend citation) -
- Muller M, et al. 1993. EMBO J. 12:4221
- Product Citations
- RRID
-
AB_2749867 (BioLegend Cat. No. 603701)
AB_2749868 (BioLegend Cat. No. 603702)
Antigen Details
- Structure
- STAT1α is a 750 amino acid protein with a predicted molecular weight of 87 kD; contains an SH2 domain responsible for homodimerization or heterodimerization.
- Distribution
-
Ubiquitously expressed; cytosolic and nuclear distribution
- Function
- Transcription activator/Cytokine Signaling
- Interaction
- Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Durbin JE, et al. 1996. Cell 8:443.
- Darnell JE Jr, et al. 1994. Science 264:1415.
- Chen X, et al. 1998. Cell 93:827.
- Ramana CV, et al. 2000. Oncogene 19:2619.
- Gene ID
- 6772 View all products for this Gene ID
- UniProt
- View information about STAT1 on UniProt.org
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