- Clone
- A15115A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP), PARK1, NACP, PD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15115A_HRP_alpha-synuclein_Antibody_1_022819_updated.png A15115A_HRP_alpha-synuclein_Antibody_1_022819_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15115A_HRP_alpha-synuclein_Antibody_1_022819_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Western blot of HRP anti-α-Synuclein, 80-96 antibody (clone A15115A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of human frontal cortex lysate; Lane 4: 20 µg of mouse brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody for 60 minutes at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. -
IHC staining of HRP anti-α-Synuclein, 80-96 antibody (clone A15115A) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson's disease (right panel) human brain tissues. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissues were incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Save |
---|---|---|---|
848303 | 25 µg |
Item is currently unavailable |
|
848304 | 100 µg |
Item is currently unavailable |
Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-Conjugated synthetic peptide that included amino acid residues 80-96 of human α-synuclein.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not cross-react with ß or ?-synucleins using ELISA and WB as detection method.
- RRID
-
AB_2734622 (BioLegend Cat. No. 848303)
AB_2734623 (BioLegend Cat. No. 848304)
Antigen Details
- Structure
- α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
- Distribution
-
Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular. - Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein, Phospho-Proteins
- Antigen References
-
1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed
2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed
3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed - Gene ID
- 6622 View all products for this Gene ID
- UniProt
- View information about alpha-Synuclein 80-96 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All α-Synuclein, 80-96 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-α-Synuclein, 80-96 | A15115A | IHC-P,Direct ELISA,WB |
HRP anti-α-Synuclein, 80-96 | A15115A | WB,IHC-P |
Biotin anti-α-Synuclein, 80-96 | A15115A | WB,IHC-P |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-α-Synuclein, 80-96
IHC staining of purified anti-α-Synuclein, 80-96 antibody (c... Western blot of purified anti-α-Synuclein, 80-96 antibody (c... Direct ELISA of purified anti-α-Synuclein antibody, 80-96 (c... -
HRP anti-α-Synuclein, 80-96
Western blot of HRP anti-α-Synuclein, 80-96 antibody (clone ... IHC staining of HRP anti-α-Synuclein, 80-96 antibody (clone ... -
Biotin anti-α-Synuclein, 80-96
Western blot of Biotin anti-α-Synuclein, 80-96 antibody (clo... IHC staining of Biotin anti-α-Synuclein, 80-96 antibody (clo...
Follow Us