- Clone
- W16030C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nitric oxide synthase 2, nitric oxide synthase 2A, NOS2A, HEP-NOS, inducible nitric oxide synthase (iNOS), Nitric oxide synthase, inducible, Inducible NOS
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16030C_PE_Nos2_Antibody_081320 W16030C_PE_Nos2_Antibody_081320](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16030C_PE_Nos2_Antibody_081320.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Thioglycolate-elicited Balb/c mouse peritoneal macrophages were incubated overnight with (left) and without (right) LPS. Cells were then surface stained with CD11b APC before being fixed with Fixation Buffer and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Finally cells were stained with anti-Nos2 (iNOS) (clone W16030C) PE.
Cat # | Size | Price | Save |
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696805 | 25 µg | ¥33,660 | |
696806 | 100 µg | ¥87,120 |
Nitric Oxide Synthase 2 (NOS2), also known as inducible NOS (iNOS), contains an N-terminal oxygenase domain and a C-terminal reductase domain, and functions to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a reactive free radical which acts as a biologic messenger with diverse functions throughout the body, such as neurotransmission, antimicrobial, and antitumor activity. NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. NOS2 also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such as COX2.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse NOS2 recombinant protein (1-250 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Mouse NOS2 has two isoforms: Isoform A is 130 kD and isoform B is 118 kD. This clone recognizes isoform B better than A in the tested cell.
- Product Citations
-
- RRID
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AB_2876745 (BioLegend Cat. No. 696805)
AB_2876745 (BioLegend Cat. No. 696806)
Antigen Details
- Structure
- 1144 amino acids with a predicted molecular weight of 130 kD. Contains a flavodoxin-like domain and a FAD-binding FR-type domain.
- Distribution
-
Cytoplasm
- Function
- NOS2 is a nitric oxide synthase and produces large quantity of NO in response to the stimulation of proinflammatory cytokines. NOS2 plays an important role in host defense against pathogens and participates in anti-tumor processes.
- Interaction
- Forms a homodimer. Interacts with SLC9A3R1, GAPDH, S100A8, and S100A9.
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Antigen References
-
1. Salim T, et al. 2016. PLoS One. 11:e0153289.
2. Ma Z, et al. 2016. Inflammation. 39:543.
3. Lee SH, et al. 2016. J. Cell. Biochem. 117:978.
4. Landes MB, et al. 2015. J. Leuko. Biol. 97:1111.
5. Karpuzoglu E, et al. 2006. Nitric. Oxide 15:177.
6. Suschek CV, et al. 2004. Curr. Mol. Med. 4:763. - Gene ID
- 4843 View all products for this Gene ID
- UniProt
- View information about Nos2 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Nos2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Nos2 (iNOS) | W16030C | WB,ICC,ICFC |
Alexa Fluor® 594 anti-Nos2 (iNOS) | W16030C | ICC |
PE anti-Nos2 (iNOS) | W16030C | ICFC |
APC anti-Nos2 (iNOS) | W16030C | ICFC |
PerCP/Cyanine5.5 anti-Nos2 (iNOS) | W16030C | ICFC |
Alexa Fluor® 647 anti-Nos2 (iNOS) | W16030C | ICFC |
PE/Cyanine7 anti-Nos2 (iNOS) | W16030C | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Nos2 (iNOS)
Total cell lysates (15 µg protein) from non-treated and LPS ... Non-treated (left image) and LPS treated (1 µg/mL for 16 hou... -
Alexa Fluor® 594 anti-Nos2 (iNOS)
Non-treated (Figure A, C and E) and LPS treated (1µg/mL, 16 ... -
PE anti-Nos2 (iNOS)
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
APC anti-Nos2 (iNOS)
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
PerCP/Cyanine5.5 anti-Nos2 (iNOS)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Alexa Fluor® 647 anti-Nos2 (iNOS)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
PE/Cyanine7 anti-Nos2 (iNOS)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w...
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