- Clone
- Poly28600 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lysozyme, LZM, 1,4-Beta-N-Acetylmuramidase C, EC 3.2.1.17, Lysozyme C, Lysozyme (Renal Amyloidosis) 2
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly28600_Purified_Lysozyme_Antibody_1_051519.png Poly28600_Purified_Lysozyme_Antibody_1_051519.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly28600_Purified_Lysozyme_Antibody_1_051519.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 10 µg of rat kidney lysate; Lane 3: 20 µg of mouse kidney lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Lysozyme is an antimicrobial enzyme that is abundantly present in secretions such as tears, saliva and mucus. Its hydrolase activity leads to the breakdown of the gram-positive bacterial cell wall and lysis of the bacteria.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant Lysozyme expressed in E.coli.
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
- Application
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WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml for human, and 1.0 - 2.0 µg per ml for mouse and rat. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2810736 (BioLegend Cat. No. 860001)
Antigen Details
- Structure
- Lysozyme is a 148 amino acid protein with an apparent molecular mass of ~ 16kD.
- Distribution
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Tissue Distribution: Spleen, kidney, milk, tears, and plasma
Cellular Distribution: Cytosol - Function
- Lysozyme is expressed in many immune cells including monocytes and macrophages. It plays an important role in innate immunity by hydrolyzing peptidoglycans found in bacterial cell wall and thus destroying the bacteria.
- Interaction
- Bacterial peptidoglycan
- Cell Type
- Macrophages, Mesenchymal cells, Monocytes, Neutrophils
- Biology Area
- Bacterial proteins and Toxins, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Enzymes and Regulators, Innate Immune Signaling, Proteases
- Antigen References
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- Mallamace D, et al. 2018. Int J Mol Sci. 19(12): E3825.
- Delorme-Axford E and Klionsky DJ. 2018. Autophagy. 14(3):365.
- Gene ID
- 4068 View all products for this Gene ID 17110 View all products for this Gene ID 25211 View all products for this Gene ID
- UniProt
- View information about Lysozyme on UniProt.org
Related FAQs
Other Formats
View All Lysozyme Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Lysozyme | Poly28600 | WB,IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Lysozyme
Western blot of purified anti-Lysozyme antibody (Poly28600).... Western blot of purified anti-Lysozyme antibody (Poly28600).... IHC staining of purified anti-Lysozyme antibody (Poly28600) ... IHC staining of purified anti-Lysozyme antibody (Poly28600) ... IHC staining of purified anti-Lysozyme antibody (Poly28600) ...
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