Recombinant Human Galectin-3 (carrier-free) (E. coli expressed)

Pricing & Availability
Regulatory Status
RUO
Other Names
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3), L31, GAL3, MAC2, Carbohydrate-Binding Protein 35 (CBP35), Galactose Binding Protein (GALBP), GALIG, Galactose-Specific Lectin 3
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Product Citations
publications
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774402 10 µg

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774404 25 µg

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774406 100 µg

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774408 500 µg

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Description

Galectin-3 is a member of the lectin family which includes 15 mammalian lectin members. Like all galectins, Galectin-3 contains a carbohydrate recognition binding domain of about 130 amino acids that enables the specific binding of β-galactosides. This protein has been shown to be involved in the cell adhesion, cell activation and chemoattraction, cell growth and differentiation, cell cycle, and apoptosis. Galectin-3 induces pro-inflammatory cytokines and inhibits Th2 type cytokine production. It chemoattracts monocytes and macrophages, and activates and degranulates basophils and mast cells. It was reported that Galectin-3 knock-out mice demonstrated reduced phagocytic properties in macrophages and Kupffer cells, indicating that this protein is involved in macrophage phagocytosis. Recently, Galectin-3 has been shown to be involved in cancer, inflammation, and fibrosis, heart disease, and stroke. It has also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, tissue repair, inflammation, fibrogenesis, and ventricular remodeling. In addition, Galectin-3 was found to contribute to the tumor cell evasion of the immune system. Galectin-3 is increasingly being used as a diagnostic marker for different cancers. It can be screened for and used as a prognostic factor to predict the progression of the cancer. Overexpression of Galectin-3 is associated with neoplastic transformation, enhancement of tumor cell adhesion to the extracellular matirx, and the subsequent metastatic spreading of the cancer cells.

Product Details
Technical data sheet

Product Details

Source
Human Galectin 3 amino acids (Ala2-Ile250) (Accession # NP_002297.2) was expressed in E. coli. The amino terminus contains MGSS6HSSGIEGR tag.
Molecular Mass
The 266 amino acid recombinant protein has a predicted molecular mass of approximately 27.8 kD. The DTT-reduced and non-reduced protein migrate at approximately 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
> 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 20 mM sodium phosphate, 0.5 M NaCl, 1 mM EDTA, 10 mM 2-Me, 5% sucrose, pH 7.4.
Endotoxin Level
Less than 1.0 EU per µg cytokine as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human Galectin-3 is able to agglutinate human red blood cells at a minimum effective concentration of 5 µg/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Zhu L, et al. 2023. J Cell Biol. :222. PubMed

Antigen Details

Structure
Monomer
Distribution

Expressed by macrophages and various organs and tissues except by the normal hepatocytes.

Function
Numerous roles in cellular functions including apoptosis, innate immunity, cell adhesion, T-cell regulation, and tumor metastasis. During IL-8 induced transmigration, Galectin-3 changed in their content and localization when neutrophils adhere to endothelia.
Interaction
Extracellular matrix
Ligand/Receptor
Binds to fibronectin, laminin, CEA, LAMPS, 90K/Mac2BP, and MP20.
Bioactivity
Human Galectin-3 is able to agglutinate human red blood cells.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family
Adhesion Molecules
Antigen References
  1. Hadari YR, et al. 2000. J. Cell. Sci. 113:2385-97.
  2. Dawson H, et al. 2013. Anticancer Res. 33:3053.
  3. Newlaczyl AU, Yu LG. 2011. Cancer Lett. 313:123.
  4. deFilippi CR, Seliger SL. 2009. J. Am. Coll. Cardiol. 54:365-7.
  5. Henderson N, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:5060.
  6. Sano H, et al. 2000. J. Immunol. 165:2156.
  7. Gao P, et al. 2013. Respir. Res. 14:136.
  8. Gil CD, et al. 2006. Cell Biol. Int. 30:338.
Gene ID
3958 View all products for this Gene ID
UniProt
View information about Galectin-3 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 06/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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