- Clone
- O92B6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transcription factor SOX-17, SOX-17, SOX 17, SRY-Box17, SRY-Related HMG-Box Transcription Factor SOX17.
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![O92B6_PURE_SOX17_1_WB_121019 O92B6_PURE_SOX17_1_WB_121019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/O92B6_PURE_SOX17_1_WB_121019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from HeLa (negative control) and OVCAR-3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Sox17 antibody, clone O92B6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 643807) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
OVCAR3 cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:200 diluted (2.5µg/mL), anti-SOX17 (clone O92B6), overnight at 4°C followed by 1:200 diluted (2.5µg/mL) Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG for anti-SOX17antibody, for one hour at room temperature. Actin filaments were labeled with Flash Phalloidin™ Green 488 (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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SOX17 is an important member of the SOX family, which is involved in various developmental processes including germ layer formation, cell-type specification, and organogenesis. SOX17 activates endodermal target genes, and is required for the formation of endoderm and blood vessel endothelium. SOX17 also plays critical roles in the maintenance of fetal and neonatal hematopoietic stem cells.
SOX17 is also proved to be a tumor suppressor through suppression of Wnt signaling in gastrointestinal carcinomas. SOX17 expression is down-regulated in colon cancer cells by methylating promoter, and the reduced expression of SOX17 in colon cancer cells mitigates the efficiency of the colony formation.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein expressed in E. Coli corresponding to aa 177-414 of human SOX17.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL (1:1000 - 1:250 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL (1:500 - 1:100 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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Antigen Details
- Structure
- 414 amino acids, and predicted Mw is 44kDa.
- Distribution
-
Nucleus
- Function
- Acts as transcription regulator that binds target promoter DNA and bends the DNA.
- Interaction
- Interacts with CTNNB1, LEF1 and TCF4.
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Antigen References
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1. Wegner M. 1999. Nucleic Acids Res. 27:1409.
2. Du YC, et al. 2009. Gastroenterology 137:1346.
3. Zorn AM, et al. 1999. Mol. Cell. 4:487.
4. Sinner D, et al. 2004. Development 131:3069.
5. Hudson C, et al. 1997. Cell. 91:397.
6. Matsui T, et al. 2006. J. Cell. Sci. 119:3513.
7. Kim I, et al. 2007. Cell. 130:470.
8. Zhang W, et al. 2008. Cancer Res. 68:2764. - Gene ID
- 64321 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- SOX17
- Specificity Alt (DOES NOT SHOW ON TDS):
- SOX17
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ICC
- UniProt
- View information about SOX17 on UniProt.org
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