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- Clone
- A20017A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PRKAA1, PRKAA2, AMPKa1, AMPKa2, AMPK, ACACA Kinase, Tau-Protein Kinase PRKAA1, Tau-Protein Kinase PRKAA2, Protein kinase AMP-Activated, Alpha 1 Catalytic Subunit, HMGCR Kinase
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
HEK293 cells untreated (negative control, open histogram) or treated with oligomycin (positive control, filled histogram) were fixed and permeabilized with the True-Phos™ Perm Buffer (Cat. No. 425401), and then intracellularly stained with Alexa Fluor® 488 anti-AMPKα Phospho (Thr172) (clone A20017A) or Alexa Fluor® 488 mouse IgG1, κ isotype control (open histogram, dashed line) (Cat. No. 400133).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 600653 | 25 tests | 144€ | ||||
| 600654 | 100 tests | 360€ | ||||
AMP-activated protein kinase alpha (AMPKα) is one subunit of the αβγ heterotrimeric protein complex AMPK. It is a key regulator of metabolism and energy homeostasis in eukaryotes through induction of catabolic pathways and deactivation of anabolic. As an inhibitor of mammalian target of rapamycin complex 1 (mTORC1) pathway, AMPKα is involved in the regulation of cellular growth, cell cycle progression, autophagy, and may play dual roles as both tumor suppressor and promotor. AMPKα is activated via phosphorylation of Threonine residue 172 (Thr172) within the activation loop of the α subunit and is promoted by the allosteric binding of AMP and/or ADP to AMPK’s γ subunit. This phosphorylation is mediated by the serine/threonine kinase liver kinase B1 (LKB1) in conjunction with accessory subunits STRAD and MO25 and also by calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2 aka CAMKKβ). Activation is induced by increases in cellular AMP:ATP and ADP:ATP ratios following a decline in ATP levels as well as by cellular stress, DNA damage, glucose starvation, and fluxes in calcium and nutrient levels. Localization of AMPKα Thr172 at the spindle poles suggests a novel role for AMPK in mitotic spindle orientation. AMPKα is a key therapeutic target in the treatment of metabolic disorders and an emerging potential treatment target for cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of human AMPK alpha phosphorylated at Thr172
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
Antigen Details
- Structure
- AMPKα is a 552 amino acid protein with a predicted molecular weight of 63 kD.
- Distribution
-
Heart, Skeletal muscle, Kidney / Nucleus and cytoplasm
- Function
- Serine/threonine-protein kinase, transcription regulation, Wnt signaling, metabolism
- Ligand/Receptor
- ATP-binding, magnesium, Metal-binding, Nucelotide-binding
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
- Mihaylova MM, et al. 2011. Nat Cell Biol 13:1016-23.
- Stein SC, et al. 2000. Biochem J. 345:437-43.
- Thaiparambil JT, et al. 2012. Mol Cell Biol. 16:3203-17.
- Vara-Ciruelos D, et al. 2019. Open Biol. 9:190099.
- Willows R, et al. 2017. Biochem J. 474:3059-3073.
- Gene ID
- 5563 View all products for this Gene ID 5562 View all products for this Gene ID
- UniProt
- View information about AMPKalpha Phospho Thr172 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All AMPKα Phospho (Thr172) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-AMPKα Phospho (Thr172) | A20017A | WB,ICC,ICFC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-AMPKα Phospho (Thr172)
Whole cell extracts (15 µg total protein) from serum-starved... 
Whole cell extracts (15 µg total protein) from untreated C2C... 
Serum-starved untreated HEK293 cells (negative control, pane... 
HEK293 cells were either untreated (negative control, open h... 
HC staining of Purified anti-AMPKα Phospho (Thr172) on...
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