- Clone
- NC-08 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![NC-08_A594_Popoplanin_Antibody_102820 NC-08_A594_Popoplanin_Antibody_102820](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/NC-08_A594_Popoplanin_Antibody_102820.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human glioblastoma cell line, LN319 were fixed with 1% paraformaldehyde (PFA) for ten minutes then blocked by 5% FBS for 30 minutes. The tissue was then stained with 10 µg/mL of Podoplanin (clone NC-08) Alexa Fluor® 594 (red) overnight at 4°C in the dark. The nuclei were then counterstained with DAPI (blue). The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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337018 | 100 µg | 292€ |
Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence1.
- Application References
-
- Fujino N, et al. 2012. Am. J. Respir. Cell. Mol. Biol. 46:422. (FC, IF)
- RRID
-
AB_2629590 (BioLegend Cat. No. 337018)
Antigen Details
- Structure
- 40-43 kD type-I transmembrane sialomucin-type glycoprotein
- Distribution
-
Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells
- Function
- Lymphatic vessel formation, tumor metastasis, platelet aggregation
- Ligand/Receptor
- CLEC2
- Cell Type
- Dendritic cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
1. Raica M, et al. 2008. Anticancer Res. 28:2997.
2. Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
3. Ogasawara S, et al. 2008. Hybridoma. 27:259.
4. Kato Y, et al. 2003. J. Bio. Chem. 278:51599. - Gene ID
- 10630 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
Other Formats
View All Podoplanin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Podoplanin | NC-08 | FC,IHC-P,ICC,SB |
PE anti-human Podoplanin | NC-08 | FC |
Alexa Fluor® 647 anti-human Podoplanin | NC-08 | FC |
Alexa Fluor® 488 anti-human Podoplanin | NC-08 | FC |
PE/Cyanine7 anti-human Podoplanin | NC-08 | FC |
Biotin anti-human Podoplanin | NC-08 | FC |
PerCP/Cyanine5.5 anti-human Podoplanin | NC-08 | FC |
Alexa Fluor® 594 anti-human Podoplanin | NC-08 | ICC |
TotalSeq™-A0127 anti-human Podoplanin | NC-08 | PG |
APC/Cyanine7 anti-human Podoplanin | NC-08 | FC |
APC/Fire™ 750 anti-human Podoplanin | NC-08 | FC |
FITC anti-human Podoplanin | NC-08 | FC |
APC anti-human Podoplanin | NC-08 | FC |
PE/Dazzle™ 594 anti-human Podoplanin | NC-08 | FC |
TotalSeq™-C0127 anti-human Podoplanin | NC-08 | PG |
TotalSeq™-B0127 anti-human Podoplanin | NC-08 | PG |
TotalSeq™-D0127 anti-human Podoplanin | NC-08 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Podoplanin
SeqIF™ (sequential immunofluorescence) staining on COMET™ of... IHC staining of Purified anti-human Podoplanin (clone NC-08)... IHC staining of Purified anti-human Podoplanin (clone NC-08)... -
PE anti-human Podoplanin
Human glioblastoma cell line LN319 stained with NC-08 PE -
Alexa Fluor® 647 anti-human Podoplanin
Human glioblastoma cell line LN319 stained with NC-08 Alexa ... -
Alexa Fluor® 488 anti-human Podoplanin
Human glioblastoma cell line LN 319 stained with NC-08 Alexa... -
PE/Cyanine7 anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
Biotin anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with biotinyl... -
PerCP/Cyanine5.5 anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
Alexa Fluor® 594 anti-human Podoplanin
Human glioblastoma cell line, LN319 were fixed with 1% paraf... -
TotalSeq™-A0127 anti-human Podoplanin
-
APC/Cyanine7 anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
APC/Fire™ 750 anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
FITC anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
APC anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
PE/Dazzle™ 594 anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... -
TotalSeq™-C0127 anti-human Podoplanin
-
TotalSeq™-B0127 anti-human Podoplanin
-
TotalSeq™-D0127 anti-human Podoplanin
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