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Brilliant Violet 421™ anti-GATA3 Antibody

Pricing & Availability
Clone
W19195B (See other available formats)
Regulatory Status
RUO
Other Names
GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19195B_BV-421_GATA3_1_030524
Murine Th2 cell line D10.G4.1 was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) Brilliant Violet 421™ (filled histogram), or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • W19195B_BV-421_GATA3_1_030524
    Murine Th2 cell line D10.G4.1 was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) Brilliant Violet 421™ (filled histogram), or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • W19195B_BV-421_GATA3_2_030524
    Th2 polarized human peripheral blood lymphocytes were surface stained with anti-human CD3 (clone UCHT1) APC. Cells were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) Brilliant Violet 421™ (left), or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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386911 25 tests 144€
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386912 100 tests 356€
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Description

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines. This is responsible for the preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defects in GATA3 result in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human GATA3 recombinant protein (1-258 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone W19195B does not block 16E10A23, TWAJ, or L50-823.

Antigen Details

Structure
48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus
Distribution

Nucleus

Function
GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T) GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation
Interaction
Interacts with Smad3, FOG1, FOG2, and PU.1
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Yagi R, et al. 2011. Int Immunol. 23:415-20.
  2. Chou J, et al. 2010. J Cell Physiol. 222:42-9.
  3. Ho IC, et al. 2009. Nat Rev Immunol. 9:125-35.
  4. Rothenberg EV, et al. 2008. Semin Immunol. 20:236-46.
  5. Kouros-Mehr H, et al. 2008. Curr Opin Cell Biol. 20:164-70.
  6. Van Esch H, et al. 2000. Nature. 406:419-22.
Gene ID
2625 View all products for this Gene ID
UniProt
View information about GATA3 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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