LEGENDplex™ Hu Tumor-Assoc. Macrophage Panel (13-plex) w/ FP

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Regulatory Status
RUO
Other Names
MMP-9, CXCL-9 (MIG), CCL-18 (PARC), CD163, MMP-2, Galectin-3 (LGALS3), CCL-20 (MIP-3α), VEGF, M-CSF, PDGF-BB, G-CSF, CCL-22 (MDC), YKL-40 (CHI3L1)
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741447 100 tests 1409€
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Description

Macrophages are produced by differentiation of monocytes in response to an infection or tissue damage. They play important roles in the immune response and in the maintenance of tissue homeostasis, such as recognizing, engulfing, destroying, and clearing foreign or harmful substances including pathogens, dying or dead cells, cellular debris and tumor cells. Many tumors recruit monocytes from circulation and transform them into an immunosuppressive subset called Tumor-Associated Macrophages (TAMs). TAMs are highly plastic and can alter their phenotype between M1 pro-inflammatory and M2 anti-inflammatory according to their location and surrounding cytokine milieu in the tumor microenvironment. M1 macrophages are considered to be functioning in the recognition and destruction of cancer cells whereas M2 macrophages are thought to promote proliferation, angiogenesis, and metastasis of cancer cells. Therefore, the phenotypes of TAMs and the soluble factors produced by them influence various aspects of cancer progression and patient prognosis. 

The LEGENDplex™ Human Tumor-Associated Macrophage Panel (13-plex) is a bead-based multiplex assay, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 key targets involved in monocyte differentiation and macrophage functions including MMP-9, CXCL-9, CCL18, CD163, MMP-2, Galectin-3, CCL20 (MIP-3α), VEGF, M-CSF, PDGF-BB, G-CSF, CCL22 (MDC), and YKL-40 (CHI3L1).  This assay panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads 
  • Setup Beads: Raw Beads 
  • LEGENDplex™ Human Tumor-Associated Macrophage Panel Premixed Beads
  • LEGENDplex™ Human Tumor-Associated Macrophage Panel Detection Antibodies 
  • LEGENDplex™ Human Tumor-Associated Macrophage Panel Standard
  • LEGENDplex™ Matrix C, Lyophilized
  • LEGENDplex™ SA-PE 
  • LEGENDplex™ Assay Buffer 
  • LEGENDplex™ Wash Buffer, 20X
  • Plate Sealers 
  • Filter Plate

Product Details

Verified Reactivity
Human
Application

Multiplex - Panel


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes 
  • Polypropylene Microfuge Tubes
  • Vortex Mixer 
  • Sonicator
  • Aluminum Foil
  • Absorbent Pads or Paper Towels 
  • Plate Shaker
  • Tabletop Centrifuges
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
  • 1.1 mL polypropylene micro FACS tubes, in 96-tube rack

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 1    Revision Date: 03/05/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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