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MojoSort™ Human anti-PE Nanobeads

Pricing & Availability
Regulatory Status
RUO
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Product Citations
publications
Mojosort_Human_PE_Nanobeads_1_082317
PBMCs were prepared and stained with CD19 (clone HIB19) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_1_082317
    PBMCs were prepared and stained with CD19 (clone HIB19) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_2_082317
  • Mojosort_Human_PE_Nanobeads_3_082317
    PBMCs were prepared and stained with TCR Vγ9 (clone B3) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_4_082317
  • Mojosort_Human_PE_Nanobeads_5_082317
    PBMCs were prepared and stained with CD3 (clone UCHT1) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_6_082317
Cat # Size Price Quantity Check Availability Save
480091 100 µL 180€
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480092 1 mL 528€
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Description

Human PE labeled PBMCs are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-human PE-conjugated antibody, followed by incubating with directly conjugated anti-PE Nanobeads. The PE+, magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the PE+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480091:

  • 1 vial containing 100 µL of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 1 vial containing 100 µL of Human anti-PE Nanobeads

For Cat# 480092:

  • 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 1 vial containing 1 ml of Human anti-PE Nanobeads

Product Details

Verified Reactivity
Human
Formulation
Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

Volume of MojoSort™ Human anti-PE Nanobeads should be adjusted depending on starting percentage of cell type to be isolated. For 1x107 cells in 100 µl of buffer, use the following volumes:

Cell type

Cell Frequency in PBMCs

Volume of Nanobeads (for 1x107 cells in 100 µl of buffer)

CD3

50-60%

20 µl

CD4 (including monocytes)

50-55%

20 µl

CD8a (including low expressing cells)

15-20%

10 µl

CD19

5-10%

4 µl

TCR Vγ9

1-3%

2 µl

 

Always use 5 µL Human TruStain for 1x107 cells in 100 µl of buffer. If additional vials of TruStain are required, please order Cat: 422301 or 422302.  

 

For low frequency cells, pre-dilute the Nanobeads in order to pipette a minimum of 5 µl of any solution. For example, to isolate TCR Vγ9+ cells, pre-dilute 10 µl of PE Nanobeads in 40 µl of MojoSort™ buffer and add 10 µl of that dilution per sample. Avoid working with small volumes.

Application Notes

The MojoSort™ Human anti-PE Nanobeads are designed to be used with BioLegend PE (phycoerythrin) conjugated antibodies against human cells. It can be used as a two-step kit for the positive selection or depletion of PE+ labeled cells from peripheral blood mononuclear cells (PBMCs).

 

Product Citations
  1. Schmitz AJ, et al. 2021. Immunity. 54:2159. PubMed

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 07/27/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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