- Clone
- BM16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BM16_PB_CD294_Antibody_071118 BM16_PB_CD294_Antibody_071118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BM16_PB_CD294_Antibody_071118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD194 (CCR4) PE, and CD294 (clone BM16) Pacific Blue™ (left) or rat IgG2a, κ Pacific Blue™ isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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350129 | 25 tests | 95€ | ||||
350130 | 100 tests | 244€ |
CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Rat cell line TART/B19-12.10 transfected with human CRTH2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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This product is sold under license agreement with BML, Inc for research use only.
- Application References
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- Nagata K, et al. 1999. J. Immunol. 162:1278. (FC)
- De Fanis U, et al. 2007. Blood 109:4343. (FC)
- Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC)
- Hamada K, et al. 2004. Allergology International 53:179. (FC)
- Product Citations
-
- RRID
-
AB_2750056 (BioLegend Cat. No. 350129)
AB_2750057 (BioLegend Cat. No. 350130)
Antigen Details
- Structure
- Seven-transmembrane protein coupled with G proteins
- Distribution
-
Effector T helper 2 (Th2) cells, eosinophils, basophils
- Function
- Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
- Interaction
- Heterotrimeric G proteins
- Ligand/Receptor
- Prostaglandin D2
- Cell Type
- Th2, Eosinophils, Basophils
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792. - Gene ID
- 11251 View all products for this Gene ID
- UniProt
- View information about CD294 on UniProt.org
Other Formats
View All CD294 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD294 (CRTH2) | BM16 | FC |
Alexa Fluor® 647 anti-human CD294 (CRTH2) | BM16 | FC |
PE anti-human CD294 (CRTH2) | BM16 | FC |
FITC anti-human CD294 (CRTH2) | BM16 | FC |
APC anti-human CD294 (CRTH2) | BM16 | FC |
Brilliant Violet 421™ anti-human CD294 (CRTH2) | BM16 | FC |
APC/Cyanine7 anti-human CD294 (CRTH2) | BM16 | FC |
PerCP/Cyanine5.5 anti-human CD294 (CRTH2) | BM16 | FC |
PE/Cyanine7 anti-human CD294 (CRTH2) | BM16 | FC |
Brilliant Violet 510™ anti-human CD294 (CRTH2) | BM16 | FC |
Brilliant Violet 605™ anti-human CD294 (CRTH2) | BM16 | FC |
Brilliant Violet 711™ anti-human CD294 (CRTH2) | BM16 | FC |
PE/Dazzle™ 594 anti-human CD294 (CRTH2) | BM16 | FC |
TotalSeq™-A0102 anti-human CD294 (CRTH2) | BM16 | PG |
Pacific Blue™ anti-human CD294 (CRTH2) | BM16 | FC |
TotalSeq™-C0102 anti-human CD294 (CRTH2) | BM16 | PG |
APC/Fire™ 750 anti-human CD294 (CRTH2) Antibody | BM16 | FC |
PE/Fire™ 810 anti-human CD294 (CRTH2) | BM16 | FC |
TotalSeq™-B0102 anti-human CD294 (CRTH2) | BM16 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes stained with CCR4 PerCP/C... Human Th2 polarized CD4+ T cells stained with purified BM16 ... Human Th2 polarized CD4+ T cells stained with purified BM16 ... -
Alexa Fluor® 647 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes stained with CCR4 PerCP/C... Human Th2 polarized CD4+ T cells stained with BM16 Alexa Flu... -
PE anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 APC... -
FITC anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 PE,... -
APC anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 PE,... -
Brilliant Violet 421™ anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 Per... -
APC/Cyanine7 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 Pac... -
PerCP/Cyanine5.5 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 FIT... -
PE/Cyanine7 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 Pac... -
Brilliant Violet 510™ anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 APC... -
Brilliant Violet 605™ anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 APC... -
Brilliant Violet 711™ anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 APC... -
PE/Dazzle™ 594 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 FIT... -
TotalSeq™-A0102 anti-human CD294 (CRTH2)
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Pacific Blue™ anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD194 (... -
TotalSeq™-C0102 anti-human CD294 (CRTH2)
-
APC/Fire™ 750 anti-human CD294 (CRTH2) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-B0102 anti-human CD294 (CRTH2)
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