Purified anti-Aph-1a, 245-265 (C terminus) Antibody (Previously Covance catalog# PRB-550P)

Pricing & Availability
Clone
Poly18231 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-secretase subunit APH-1A, aph-1alpha, presenilin-stabilization factor, CGI-78, anterior pharynx defective 1 homolog A, presenilin-stabilization factor
Previously
Covance Catalog# PRB-550P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
823101 100 µL 359€
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Description

The gamma secretase is a multiprotein complex consisting of presenilin, nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. While Aph-1 stabilizes the presenilin holoprotein within the gamma secretase complex, Pen-2 is required for endoproteolysis of presenilin and activation of the gamma secretase. The Aph-1 homologue on human chromosome 1 is termed Aph-1a and on human chromosome 15 is referred to as Aph-1b.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The antibody Aph-1a, 245-265 (Poly18231) was raised against the human Aph-1aL/C-terminal region and recognizes the sequence CRRQEDSRVMVYSALRIPPED of human Aph-1aL.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB, ICC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000*

Application Notes

This antibody is effective in immunoblotting (WB).

*Expected MW: 29 kD

WB Positive Control: Human Brain lysate

BioLegend also offers Poly18232 (823201) which recognizes residues 92-115 of the Aph-1aL/ S Loop.

This product may contain other non-IgG subtypes.

Application References
  1. Gu Y, Chen F, Sanjo N, Kawarai T, Hasegawa H, Duthie M, Li W, Ruan X, Luthra A, Mount HT, Tandon A, Fraser PE, St George-Hyslop P. Aph-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of the presenilin.nicastrin complexes. J Biol Chem 278:7374-7380, 2003.
Product Citations
  1. Lu J, et al. 2016. PLoS One. 11:e0166415. PubMed
RRID
AB_2564862 (BioLegend Cat. No. 823101)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Secretases
Gene ID
51107 View all products for this Gene ID
UniProt
View information about Aph-1a 245-265 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Aph-1a, 245-265 (C terminus)

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