- Clone
- P82E11C10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vav Guanine Nucleotide Exchange Factor 1, Vav 1 Guanine Nucleotide Exchange Factor, Proto-Oncogene Vav, Vav 1 Oncogene
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P82E11C10_PURE_VAV_Antibody_1_010820.png P82E11C10_PURE_VAV_Antibody_1_010820.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P82E11C10_PURE_VAV_Antibody_1_010820.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-VAV antibody (clone P82E11C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. -
Jurkat cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1.0 µg/mL (1:500 dilution) of either purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400102, panel A) or purified anti-VAV antibody (clone P82E11C10, panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. -
Whole cell extracts (250 μg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.0 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400102) or purified anti-VAV antibody (clone P82E11C10). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of VAV. Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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935101 | 25 µg | 81€ | ||||
935102 | 100 µg | 203€ |
VAV1 is one of the three members of the VAV family that functions as a guanine exchange factor (GEF) and is exclusively expressed in hematopoietic and trophoblast cells. VAV1 plays a critical role in immune synapse formation through the regulation of actin cytoskeleton organization. Upon TCR activation, VAV1 is recruited to the immunological synapse and interacts via its SH2 and C-terminal SH3 domains with phosphorylated adaptor protein SLP-76. VAV1 gets phosphorylated and undergoes conformational change to expose its DH domain that modulates GEF activity and promotes GDP to GTP exchange of Rho GTPase CDC42. CDC42 activates WASP and leads to the activation of actin polymerization. In addition, VAV1 transduces T cell receptor signaling including NFAT and NF-κB pathways in a GEF independent manner and promotes T cell activation and development.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial recombinant human VAV1 (amino acid 121-324)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Verified
Direct ELISA, FC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone does not recognize mouse VAV1.
Both methanol and Triton X-100 permeabilization produced correct staining in ICC testing. - RRID
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AB_2832888 (BioLegend Cat. No. 935101)
AB_2832889 (BioLegend Cat. No. 935102)
Antigen Details
- Structure
- VAV1 is a 845 amino acid protein with a predicted molecular weight of 98 kD.
- Distribution
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Cytosol/Hematopoeitic cells
- Function
- GEF, TCR signal transduction
- Biology Area
- Cell Biology
- Antigen References
-
- Costello P. et al. 1999. Proc Natl Acad Sci USA. 96:3035.
- Wülfing C. et al. 2000. Proc Natl Acad Sci USA. 97:10150.
- Villalba M, et al. 2001. J. Cell. Biol. 155:331.
- Tybulewicz V, et al. 2003. Immunological Reviews. 192:42.
- Katzav S. et al. 2004. Blood. 103:2443.
- Fernandez-Zapico M. et al. 2005. Cancer. Cell. 7:39.
- Gene ID
- 7409 View all products for this Gene ID
- UniProt
- View information about VAV on UniProt.org
Other Formats
View All VAV Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-VAV | P82E11C10 | WB,ICC,IP,Direct ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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