- Clone
- MHL-38 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain lambda
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The MHL-38 antibody reacts with both soluble and membrane human immunoglobulin light chain lambda (λ). It does not react with human immunoglobulin light chain kappa (κ) or heavy chains. The MHL-38 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone MHL-38 is not compatible with Human TruStain FcX (Fc Receptor Blocking Solution) (Cat. No. 422301 & 422302).
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3535 View all products for this Gene ID
- UniProt
- View information about Ig light chain lambda on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All Ig light chain λ Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ig light chain λ
Over night cultured human peripheral blood lymphocytes stain... IHC staining using purified anti-human Ig light chain λ anti... -
Biotin anti-human Ig light chain λ
-
FITC anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 FITC Human peripheral blood lymphocytes stained with MHL-38 FITC -
PE anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 PE -
APC anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 PE and ... -
Alexa Fluor® 488 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 Alexa... -
Alexa Fluor® 647 anti-human Ig light chain λ
Human peripheral blood lymphoctes stained with MHL-38 Alexa ... -
Pacific Blue™ anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 PE and ... -
PerCP/Cyanine5.5 anti-human Ig light chain λ
Human peripheral blood lymphocytes were stained with CD19 PE... -
Purified anti-human Ig light chain λ (Maxpar® Ready)
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 151Eu-a... -
PE/Dazzle™ 594 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 Brillia... -
PE/Cyanine7 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 Brillia... -
APC/Fire™ 750 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 Brillia... -
TotalSeq™-A0898 anti-human Ig light chain λ
-
TotalSeq™-C0898 anti-human Ig light chain λ
-
Alexa Fluor® 700 anti-human Ig light chain λ
Human peripheral blood lymphocytes were costained with CD19 ... -
TotalSeq™-B0898 anti-human Ig light chain λ
-
APC/Cyanine7 anti-human Ig light chain λ
Human peripheral blood lymphocytes were stained with CD19 FI... -
TotalSeq™-D0898 anti-human Ig light chain λ
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TotalSeq™-Bn0898 anti-human Ig light chain λ
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Spark Red™ 718 anti-human Ig light chain λ (Flexi-Fluor™)
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PE anti-human Ig light chain λ
Typical results from overnight cultured human peripheral blo... -
APC anti-human Ig light chain λ
Typical results from overnight cultured human peripheral blo... -
APC/Fire™ 750 anti-human Ig light chain λ
Typical results from overnight cultured human peripheral blo...
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