Loading...

Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
RB6-8C5_ALX488_020708
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
  • RB6-8C5_ALX488_020708
    C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
108419 25 µg 81€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
108417 100 µg 184€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  2. Chen CC, et al. 2018. Oncoimmunology. 7:e1424612. PubMed
  3. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  4. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  5. He J, et al. 2019. J Clin Invest. 130. PubMed
  6. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  7. Hirano Y, et al. 2018. J Leukoc Biol. 104:1159. PubMed
  8. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  9. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  10. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  11. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  12. Loh Z, et al. 2020. PLOS Pathogens. 16(7):e1008651. PubMed
  13. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  14. Sasi S, et al. 2014. J Biol Chem. 289:14178. PubMed
  15. Burkhardt A, et al. 2014. J Immunol. 193:1468. PubMed
  16. Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
  17. Onishi S, et al. 2015. PLoS One. 10:126564. PubMed
  18. Nasser MW, et al. 2015. Cancer Res . 75: 974-985. PubMed
  19. Oghumu S, et al. 2014. Immunology. 143: 109-119. PubMed
  20. Krivanek J, et al. 2020. Nat Commun. 3.802777778. PubMed
  21. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  22. Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
  23. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  24. Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
  25. Rahman H, et al. 2020. J Invest Dermatol. . PubMed
  26. Magupalli VG, et al. 2020. Science. :369. PubMed
  27. Haas MS, et al. 2020. Mol Cancer Res. 19:717. PubMed
  28. Huang YL, et al. 2021. Commun Biol. 4:229. PubMed
  29. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  30. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  31. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  32. Boltersdorf T, et al. 2020. Theranostics. 10:6599. PubMed
  33. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  34. Hsing CH, et al. 2021. Biomedicines. 9:. PubMed
  35. Higa T, et al. 2022. Nat Commun. 13:1500. PubMed
  36. Cruz MA, et al. 2022. Nat Nanotechnol. 17:1004. PubMed
  37. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  38. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  39. Li J, et al. 2022. Nat Commun. 13:1481. PubMed
  40. Liu Y, et al. 2022. iScience. 25:105121. PubMed
  41. Sklepkiewicz P, et al. 2023. Int J Mol Sci. 24:. PubMed
  42. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
RRID
AB_493480 (BioLegend Cat. No. 108419)
AB_389309 (BioLegend Cat. No. 108417)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account