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Alexa Fluor® 647 anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI30_Alx647_071307
Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 647
  • HI30_Alx647_071307
    Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
304020 25 tests 90€
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304056 100 µg 198€
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304018 100 tests 212€
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Futia G, et al. 2017. Cytometry A. 10.1002/cyto.a.23144. PubMed
  2. Chen S, et al. 2017. PLoS One. 10.1371/journal.pone.0181249. PubMed
  3. Olah M, et al. 2018. Nat Commun. 9:539. PubMed
  4. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  5. Garcia J, et al. 2019. Cancer Med. 10.1002/cam4.2244. PubMed
  6. Gonzales–Aloy E, et al. 2019. Haematologica. 104:296. PubMed
  7. Janning M, et al. 2019. Cancers (Basel). 11. PubMed
  8. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  9. Luong-Gardiol N, et al. 2019. Cancer Cell. 35:649. PubMed
  10. Aguilar–Avelar C, et al. 2019. Sci Rep. 9:13766. PubMed
  11. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  12. Razvag Y, et al. 2019. Cell Rep. 29:3506. PubMed
  13. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  14. Yee–de León JF, et al. 2020. Sci Rep. 5.654861111. PubMed
  15. Perry S, et al. 2011. PLoS One. 7:e1001297. PubMed
  16. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  17. Lim H, et al. 2015. J Immunol. 195: 5432 - 5439. PubMed
  18. McComb S, et al. 2016. Sci Transl Med. 8: 339ra70. PubMed
  19. Zhong W, et al. 2016. Nat Commun. 7: 12702. PubMed
  20. Gierahn T, et al. 2017. Nat Methods. 10.1038/nmeth.4179. PubMed
  21. Olah M, et al. 2020. Nat Commun. 4.714583333. PubMed
  22. Raimondi L, et al. 2020. Int J Mol Sci. 21:00. PubMed
  23. Bade RM, et al. 2021. Molecular Oncology. . PubMed
  24. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  25. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  26. Wohl I, et al. 2021. Front Cell Dev Biol. 9:590655. PubMed
  27. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  28. Sajman J, et al. 2021. Commun Biol. 4:439. PubMed
  29. Jung JY, et al. 2021. Cells. 10:. PubMed
  30. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  31. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  32. Staudte S, et al. 2022. Cancers (Basel). 14:. PubMed
  33. Beasley AB, et al. 2021. Methods Mol Biol. 2265:213. PubMed
  34. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  35. Patel T, et al. 2022. Aging Cell. 21:e13606. PubMed
  36. Wu HJ, et al. 2022. Cell Genom. 2: . PubMed
  37. Schwab FD, et al. 2022. Microsyst Nanoeng. 8:130. PubMed
  38. Xiao Q, et al. 2023. Methods Mol Biol. 2654:503. PubMed
RRID
AB_493034 (BioLegend Cat. No. 304020)
AB_2564155 (BioLegend Cat. No. 304056)
AB_389336 (BioLegend Cat. No. 304018)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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