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APC anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
FN50_APC_042706
PMA+ionomycin-stimulated (5hours) human peripheral blood lymphocytes stained with FN50 APC
  • FN50_APC_042706
    PMA+ionomycin-stimulated (5hours) human peripheral blood lymphocytes stained with FN50 APC
See APC spectral data
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310909 25 tests 58€
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310910 100 tests 124€
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Application References
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  8. Lee YG, et al. 2019. Nat Commun. 10:2681. PubMed
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  36. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
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  38. O'Donoghue GP, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
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  47. Liu Y, et al. 2022. Biochem J. 479:385. PubMed
  48. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  49. Li Q, et al. 2022. J Biol Chem. :101753. PubMed
  50. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  51. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  52. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  53. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  54. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  55. Gudgeon N, et al. 2022. Cell Rep. 40:111193. PubMed
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  58. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
  59. Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed
  60. Huang M, et al. 2021. BMC Infect Dis. 21:922. PubMed
  61. Xiao C, et al. 2022. iScience. 25:103934. PubMed
  62. Kuloglu ZE, et al. 2022. Allergy. 77:2459. PubMed
  63. Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed
  64. Rosato F, et al. 2022. J Transl Med. 20:578. PubMed
  65. Mamula D, et al. 2022. NPJ Parkinsons Dis. 8:171. PubMed
  66. Kerns JS, et al. 2023. Bio Protoc. 13: . PubMed
  67. Leick MB, et al. 2022. Cancer Cell. 40:494. PubMed
  68. Duan LJ, et al. 2022. Cell Rep. 40:111284. PubMed
  69. Felce SL, et al. 2021. Wellcome Open Res. 5:42. PubMed
  70. Anderson W, et al. 2023. Elife. 12:. PubMed
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RRID
AB_314844 (BioLegend Cat. No. 310909)
AB_314845 (BioLegend Cat. No. 310910)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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