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APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
RB6-8C5_APCCyanine7_Ly6G-Ly6C_Antibody_020119
C57BL/6 mouse bone marrow stained with CD11b FITC and Ly-6G/Ly-6C (Gr-1) (clone RB6-8C5) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right). Dot plots are gated on myeloid cell population).
  • RB6-8C5_APCCyanine7_Ly6G-Ly6C_Antibody_020119
    C57BL/6 mouse bone marrow stained with CD11b FITC and Ly-6G/Ly-6C (Gr-1) (clone RB6-8C5) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right). Dot plots are gated on myeloid cell population).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
108423 25 µg 81€
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108424 100 µg 186€
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  2. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  3. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  4. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  5. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  6. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  7. Mayers S, et al. 2019. BMC Biotechnol. 19:21. PubMed
  8. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  9. Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
  10. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  11. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  12. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  13. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  14. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  15. Lin W, et al. 2019. EBioMedicine. 49:133. PubMed
  16. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  17. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  18. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  19. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  20. Jaitin DA, et al. 2020. Cell. 178(3):686-698.e14.. PubMed
  21. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  22. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  23. Firas J, et al. 2014. Differentiation. 87:193. PubMed
  24. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  25. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  26. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  27. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  28. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  29. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  30. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  31. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  32. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  33. Liu Z, et al. 2021. Immunity. 54(2):247-258.e7. PubMed
  34. Young K, et al. 2021. Cell Stem Cell. . PubMed
  35. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  36. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  37. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  38. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  39. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  40. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  41. Gu H, et al. 2021. Immun Inflamm Dis. 9:1686. PubMed
  42. Deets KA, et al. 2021. Elife. 10:. PubMed
  43. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  44. Xu G, et al. 2022. Front Pharmacol. 13:896601. PubMed
  45. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  46. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  47. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  48. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  49. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  50. Denny L, et al. 2021. Clin Transl Immunology. 10:e1234. PubMed
  51. Bregolat NF, et al. 2022. Haematologica. 107:2454. PubMed
  52. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  53. Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed
  54. Solier S, et al. 2023. Int J Mol Sci. 24:. PubMed
  55. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  56. Zhang H, et al. 2023. Pharmaceutics. 15:. PubMed
RRID
AB_2137486 (BioLegend Cat. No. 108423)
AB_2137485 (BioLegend Cat. No. 108424)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 02/01/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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