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Pacific Blue™ anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_Blue_022008
Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
  • HIB19_Blue_022008
    Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
302223 25 µg 100€
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302232 100 tests 212€
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302224 100 µg 212€
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Request Bulk Quote
Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤ 0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Chen YQ et al. 2018. Cell. 173(2):417-429 . PubMed
  2. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  3. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  4. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  5. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  6. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  7. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  8. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  9. Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed
  10. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  11. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  12. Rius C, et al. 2018. J Immunol. 200:2263. PubMed
  13. Longino NV, et al. 2019. Cancer Immunol Res. 7:1727. PubMed
  14. Darcis G, et al. 2020. Cell Rep. 30:2284. PubMed
  15. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  16. Koning D, et al. 2013. J Immunol. 190:391. PubMed
  17. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  18. Bartholomaeus P, et al. 2014. J Immunol. 192:2091. PubMed
  19. Steinsbø &, et al. 2014. Nat Commun. 5:4041. PubMed
  20. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
  21. Boer M, et al. 2015. Clin Vaccine Immunol. 22: 778 - 788. PubMed
  22. Linley A, et al. 2015. Blood. 126: 1902 - 1910. PubMed
  23. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed
  24. Della-Torre E, et al. 2015. Ann Rheum Dis. 74: 2236 - 2243. PubMed
  25. Holland C, et al. 2015. J Immunol. 195: 5827 - 5836. PubMed
  26. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  27. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  28. Gauvin J, et al. 2016. PLoS One. 11: 0155868. PubMed
  29. Chen L, et al. 2016. Ann Rheum Dis. 75: 916 - 923. PubMed
  30. Malarkey C, et al. 2016. PLoS One. 11: 0157157. PubMed
  31. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  32. Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
  33. Drennan S, et al. 2016. Leukemia. 10.1038/leu.2016.356. PubMed
  34. Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
  35. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
  36. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  37. Rouers A, et al. 2021. iScience. 24:102482. PubMed
  38. Vetsika EK, et al. 2021. Cancers (Basel). 13:. PubMed
  39. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  40. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  41. Zuccato C, et al. 2022. Ther Adv Hematol. 13:20406207221100648. PubMed
  42. Dolton G, et al. 2022. Cell. 185:2936. PubMed
  43. Lindeman I, et al. 2021. J Exp Med. 218:. PubMed
  44. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  45. Song M, et al. 2022. J Gene Med. 24:e3455. PubMed
  46. Ghassemi S, et al. 2022. Nat Biomed Eng. 6:118. PubMed
  47. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
RRID
AB_493652 (BioLegend Cat. No. 302223)
AB_2073118 (BioLegend Cat. No. 302232)
AB_493653 (BioLegend Cat. No. 302224)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/18/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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