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Pacific Blue™ anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Blue_091207
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
  • 6D5_Blue_091207
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
115526 25 µg 76€
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115523 100 µg 172€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  2. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  3. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  4. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  5. Schneider C, et al. 2018. Cell. 174:271. PubMed
  6. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  7. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  8. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  9. Minguet S, et al. 2017. Nat Immunol. 18:1150. PubMed
  10. Call M, et al. 2009. J Immunol. 182:6342. PubMed
  11. Haso W, et al. 2013. Blood. 121:1165. PubMed
  12. Ding Y, et al. 2013. J Immunol. 191:1614. PubMed
  13. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  14. Dow L, et al. 2014. PLoS One. 9:95236. PubMed
  15. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  16. Dema B, et al. 2014. J Exp Med. 211:2159. PubMed
  17. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
  18. Fiala G, et al. 2015. J Exp Med. 212: 1693-1708. PubMed
  19. Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed
  20. Kajikawa A, et al. 2015. PLoS One. 10: e0141713. PubMed
  21. Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed
  22. Chen Z, et al. 2020. J Immunol. 204:335. PubMed
  23. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  24. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  25. Korin B, et al. 2020. Sleep. :43. PubMed
  26. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  27. Molgora M, et al. 2020. Cell. 182:886. PubMed
  28. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  29. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  30. Lang V, et al. 2021. Elife. 10:. PubMed
  31. Smith LK, et al. 2021. Elife. 10:. PubMed
  32. Pfeil J, et al. 2020. Front Immunol. 11:529035. PubMed
  33. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  34. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  35. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  36. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  37. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  38. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  39. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  40. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  41. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
  42. Georgiev GI, et al. 2022. Cell Chem Biol. 29:811. PubMed
RRID
AB_493341 (BioLegend Cat. No. 115526)
AB_439718 (BioLegend Cat. No. 115523)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 2    Revision Date: 05/24/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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