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PE anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_PE_060507
Human peripheral blood lymphocytes stained with OKT4 PE
  • OKT4_PE_060507
    Human peripheral blood lymphocytes stained with OKT4 PE
  • OKT4_PE_CD4_Antibody_042922
    Human peripheral blood was stained with CD4 (clone OKT4) PE (solid line) or mouse IgG2b, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
317409 25 tests 23€
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317410 100 tests 53€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Zimmerman KA, et al. 2019. Physiol Rep. 7:e13951. PubMed
  2. Li Z, et al. 2019. PLoS Pathog. 15:e1007498. PubMed
  3. Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed
  4. Dawson NA, et al. 2019. JCI Insight. 4:6. PubMed
  5. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  6. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
  7. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  8. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  9. Chang HH, et al. 2019. J Autoimmun. 101:121. PubMed
  10. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  11. Mozhgani SH, et al. 2019. Retrovirology. 16:46. PubMed
  12. Sievers C, et al. 2012. Clin Immunol. 143:162. PubMed
  13. Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed
  14. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  15. Estes J, et al. 2015. J Infect Dis. 211:744. PubMed
  16. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  17. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  18. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  19. Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
  20. Landgraf KE, et al. 2020. Commun Biol. 0.330555556. PubMed
  21. Bhargava R, et al. 2020. J Biol Chem. 295:125. PubMed
  22. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  23. Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed
  24. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  25. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  26. Bhargava R, et al. 2020. J Biol Chem. 295:125. PubMed
  27. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  28. Sitlinger A, et al. 2021. Cells. 10:. PubMed
  29. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  30. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  31. Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
  32. Cisneros-Aguirre M, et al. 2022. Nat Commun. 13:3662. PubMed
  33. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  34. Wang H, et al. 2022. iScience. 25:105065. PubMed
  35. Claeys E, et al. 2021. Front Immunol. 12:650731. PubMed
  36. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  37. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  38. Ping X, et al. 2022. DNA Repair (Amst). 119:103394. PubMed
  39. Masuda H, et al. 2022. MAbs. 14:2122275. PubMed
  40. Yi X, et al. 2023. J Med Virol. 95:e28642. PubMed
RRID
AB_571954 (BioLegend Cat. No. 317409)
AB_571955 (BioLegend Cat. No. 317410)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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