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PE/Cyanine5 anti-human CD11b Antibody

Pricing & Availability
Clone
ICRF44 (See other available formats)
Regulatory Status
RUO
Workshop
IV M047
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ICRF44_PECy5_061507
Human peripheral blood lymphocytes, monocytes, and granulocytes stained with ICRF44 PE/Cyanine5
  • ICRF44_PECy5_061507
    Human peripheral blood lymphocytes, monocytes, and granulocytes stained with ICRF44 PE/Cyanine5
See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability Save
301307 25 tests 99€
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301308 100 tests 238€
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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References
  1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  3. Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
  4. Marsik C, et al. 2003. Shock 20:493. (FC)
  5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
  6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
  8. Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
  11. Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
  12. Sprong T, et al. 2003. Blood 102:3702. (Block)
  13. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
  14. Larsson K, et al. 2015. PNAS. PubMed
Product Citations
  1. Bill M, et al. 2018. J Cell Mol Med. 22:2311. PubMed
  2. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  3. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
  4. Vaccari M, et al. 2019. Front Immunol. 10:1072. PubMed
  5. Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
  6. Girgis N, et al. 2011. J Virol. 85:2547. PubMed
  7. Bose N, et al. 2014. Glycobiology. 24:379. PubMed
  8. Sugino N, et al. 2017. Leukemia. 10.1038/leu.2017.59. PubMed
  9. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  10. Gibellini L, et al. 2020. EMBO Mol Med. 12:e13001. PubMed
  11. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  12. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  13. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  14. Sun R, et al. 2021. Cell Reports. 36(7):109559. PubMed
  15. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  16. Magg T, et al. 2021. Sci Immunol. 6:. PubMed
  17. He L, et al. 2022. EMBO Mol Med. 14:e14990. PubMed
  18. Bissa M, et al. 2023. Nat Commun. 14:575. PubMed
RRID
AB_314159 (BioLegend Cat. No. 301307)
AB_314160 (BioLegend Cat. No. 301308)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.

Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org
Go To Top Version: 4    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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