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PE Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
405307 200 µg 176€
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The IgG antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IgG antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Gu Q, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.05.011. PubMed
  2. Chittasupho C, Anuchapreeda S, and Sarisuta N. 2017. Eur J Pharm Biopharm. 10.1016/j.ejpb.2017.07.003. PubMed
  3. Vogelgesang A, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0914-6. PubMed
  4. Tsukamoto H, et al. 2017. FEBS Lett. 10.1002/1873-3468.12768. PubMed
  5. Hirata T, et al. 2018. Nat Commun. 9:405. PubMed
  6. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  7. Sakaguchi K, et al. 2018. Biochem Biophys Rep. 14:89. PubMed
  8. Vrijens P, et al. 2019. J Gen Virol. 100:583. PubMed
  9. Soday L et al. 2019. Cell reports. 27(6):1920-1933 . PubMed
  10. Wang Y, et al. 2020. Nat Commun. 11:860. PubMed
  11. Murcia JDG, et al. 2020. Sci Rep. 10:8019. PubMed
  12. Wu YF, et al. 2019. Autophagy. :1. PubMed
  13. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  14. Uray K, et al. 2011. Surgery. 149:114. PubMed
  15. Pei X, et al. 2014. J Immunol. 192:1878. PubMed
  16. Ryszawy D, et al. 2014. Carcinogenesis. 35:1920. PubMed
  17. Kimura T, et al. 2015. J Immunol. 194:5426. PubMed
  18. Gordts S, et al. 2015. PLoS One. 10: 0131219. PubMed
  19. Kmieciak M, et al. 2011. J Vis Exp. 47: 2381. PubMed
  20. Rong Y, et al. 2015. PLoS One. 10: 0138553. PubMed
  21. Hirata T, et al. 2015. Mol Biol Cell. 26: 3071-3084. PubMed
  22. Winter S, Bardoel A 2016. Sci Rep. 6: 24242. PubMed
  23. Griffiths K, et al. 2016. J Biol Chem. 291: 12641 - 12657. PubMed
  24. Tiper I, et al. 2016. Clin Cancer Res. 22: 4249 - 4258. PubMed
  25. Liu Y, et al. 2016. Acta Biochim Biophys Sin. 48: 909 - 922. PubMed
  26. Tam K, et al. 2016. MBio. 7: e02024-16. PubMed
  27. Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed
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  29. Becerril C, et al. 2021. Biomolecules. 11:. PubMed
  30. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  31. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  32. Jiang X, et al. 2021. Sci Signal. 14:. PubMed
  33. Busnadiego I, et al. 2022. PLoS Biol. 20:e3001709. PubMed
  34. Tian M, et al. 2022. J Clin Invest. 132:. PubMed
  35. Hirata T, et al. 2022. J Biol Chem. :102444. PubMed
  36. Vanhulle E, et al. 2022. Front Cell Infect Microbiol. 12:989534. PubMed
  37. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  38. Parente-Pereira AC, et al. 2022. STAR Protoc. 3:101319. PubMed
  39. Larcombe-Young D, et al. 2022. STAR Protoc. 3:101414. PubMed
  40. Petrie-Hanson L, et al. 2022. Pathogens. 11:. PubMed
  41. Byazrova MG, et al. 2022. NPJ Vaccines. 7:145. PubMed
  42. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  43. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  44. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  45. Ban B, et al. 2022. Antibodies (Basel). 11: . PubMed
  46. Im NG, et al. 2022. Cancer Immunol Res. 10:1055. PubMed
  47. Bharadwaj P, et al. 2022. Cell Rep Med. 3:100818. PubMed
  48. Ji K, et al. 2023. Clin Transl Med. 13:e1234. PubMed
  49. Daradoumis J, et al. 2023. Viruses. 15:. PubMed
  50. Zhang G, et al. 2023. J Immunother Cancer. 11:. PubMed
RRID
AB_315010 (BioLegend Cat. No. 405307)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 02/09/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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