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PE/Cyanine7 anti-human CD197 (CCR7) Antibody

Pricing & Availability
Clone
G043H7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR2, CDw197, EBI1, CMKBR7
Isotype
Mouse IgG2a, κ
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Product Citations
publications
G043H7_PECyanine7_CD197_1_050520
Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cyanine7 (left) or mouse IgG2a, κ PE/Cyanine7 isotype control (right).
  • G043H7_PECyanine7_CD197_1_050520
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cyanine7 (left) or mouse IgG2a, κ PE/Cyanine7 isotype control (right).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
353225 25 tests 147€
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353226 100 tests 306€
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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Product Citations
  1. Mukhopadhyay M, et al. 2017. J Immunol. 10.4049/jimmunol.1700953. PubMed
  2. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  3. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  4. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  5. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  6. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  7. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  8. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  9. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
  10. Valle-Casuso JC, et al. 2019. Cell Metab. 29:611. PubMed
  11. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  12. Claireaux M, et al. 2018. MBio. 9:e00317. PubMed
  13. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  14. Michaels K, et al. 2015. J Immunol. 194:3267. PubMed
  15. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  16. Gannon P, et al. 2015. J Immunol. 195: 356 - 366. PubMed
  17. Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
  18. Pillet S, et al. 2016. Clin Immunol. 168:72-87. PubMed
  19. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
  20. Chellappa S, et al. 2016. J Leukoc Biol. 100: 5 - 16. PubMed
  21. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  22. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  23. Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed
  24. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  25. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  26. Swanson E, et al. 2021. eLife. 10:00. PubMed
  27. Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed
  28. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
  29. Martins AJ, et al. 2017. Cell Systems. 4(4):379-392.e12. PubMed
  30. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  31. Schmidt J, et al. 2021. Cell Rep Med. 100194:2. PubMed
  32. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  33. Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed
  34. Fu J, et al. 2021. J Clin Invest. 131:. PubMed
  35. Stephenson E, et al. 2021. Nat Med. 27:904. PubMed
  36. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  37. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  38. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  39. Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed
  40. Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed
  41. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  42. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  43. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  44. Gyurdieva A, et al. 2022. Nat Commun. 13:5296. PubMed
  45. Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed
  46. Guo A, et al. 2022. Nature. 607:135. PubMed
  47. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  48. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  49. Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed
  50. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
RRID
AB_11125576 (BioLegend Cat. No. 353225)
AB_11126145 (BioLegend Cat. No. 353226)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 2    Revision Date: 04/08/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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