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PerCP/Cyanine5.5 anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_PerCPCyanine55_CD4_Antibody_120518
Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • RPA-T4_PerCPCyanine55_CD4_Antibody_120518
    Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
300529 25 tests 90€
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300530 100 tests 175€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. D’Antoni ML, et al. 2018. J Acquir Immune Defic Syndr. 79:108. PubMed
  2. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed
  3. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  4. Tran TM, et al. 2020. Immunity. 51(4):750-765. PubMed
  5. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  6. Sumitomo S, et al. 2013. J Immunol. 94:393. PubMed
  7. Lu T, et al. 2016. PLoS One. 11: 0148044. PubMed
  8. Beyer M, et al. 2016. Nat Immunol. 17:593-603. PubMed
  9. Zakhour R, et al. 2016. Clin Infect Dis. 62: 1029-1035. PubMed
  10. Woldemeskel BA, et al. 2020. J Clin Invest. 130:6631. PubMed
  11. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  12. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  13. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  14. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  15. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  16. Woldemeskel BA, et al. 2021. J Clin Invest. 131:. PubMed
  17. Dykema AG, et al. 2021. J Clin Invest. 131:. PubMed
  18. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  19. Wiche Salinas TR, et al. 2021. iScience. 24:103225. PubMed
  20. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  21. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  22. Woldemeskel BA, et al. 2022. J Clin Invest. 132:. PubMed
  23. Elias G, et al. 2022. Elife. 11:. PubMed
  24. Chan JA, et al. 2022. Nat Commun. 13:4159. PubMed
  25. Du L, et al. 2021. Front Mol Biosci. 8:675179. PubMed
  26. Robinson CA, et al. 2022. Viruses. 14:. PubMed
  27. Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
  28. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  29. Zheng J, et al. 2021. J Infect Dis. 223:785. PubMed
  30. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  31. Emert-Sedlak LA, et al. 2022. ACS Infect Dis. 8:91. PubMed
  32. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  33. Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed
  34. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  35. Qiu C, et al. 2022. Front Bioeng Biotechnol. 10:1027619. PubMed
  36. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  37. Gallucci L, et al. 2023. EMBO Rep. 24:e56818. PubMed
  38. Wu X, et al. 2023. iScience. 26:106559. PubMed
  39. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
RRID
AB_893328 (BioLegend Cat. No. 300529)
AB_893322 (BioLegend Cat. No. 300530)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 12/05/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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