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PerCP/Cyanine5.5 anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HI30_PerCPCyanine55_CD45_Antibody_080818
Human peripheral blood lymphocytes were stained with HI30 PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • HI30_PerCPCyanine55_CD45_Antibody_080818
    Human peripheral blood lymphocytes were stained with HI30 PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
304027 25 tests 128€
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304028 100 tests 272€
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
  2. Gerhauser C et al. 2018. Cancer cell. 34(6):996-1011 . PubMed
  3. Giraud M, et al. 2012. Proc Natl Acad Sci U S A. 109:535. PubMed
  4. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  5. Roug A, et al. 2014. Cytotherapy. 16:392. PubMed
  6. Kanderova V, et al. 2016. Mol Cell Proteomics. 15: 1246-1261. PubMed
  7. Caggiati A, et al. 2017. Aesthet Surg J. 10.1093/asj/sjw211. PubMed
  8. Soh KT, et al. 2020. Cytometry B Clin Cytom. 98:161. PubMed
  9. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  10. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  11. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  12. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  13. Xu Y, et al. 2021. Cell Reports. 36(6):109524. PubMed
  14. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  15. Imai Y, et al. 2021. JID Innov. 1:100003. PubMed
  16. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  17. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  18. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  19. Zhou L, et al. 2021. MAbs. 13:1964420. PubMed
  20. Godoy-Pacheco A, et al. 2022. Oncol Lett. 24:288. PubMed
  21. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  22. Johnson AJ, et al. 2021. Cancer Immunol Res. 9:1047. PubMed
  23. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  24. Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed
  25. Gur C, et al. 2022. Cell. 185:1373. PubMed
  26. Gao Y, et al. 2023. iScience. 26:106729. PubMed
  27. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
RRID
AB_1236444 (BioLegend Cat. No. 304027)
AB_893338 (BioLegend Cat. No. 304028)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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