- Clone
- W18195C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18195C_PURE_CD117_Antibody_070720.png W18195C_PURE_CD117_Antibody_070720.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18195C_PURE_CD117_Antibody_070720.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with purified anti-human CD117 (c-kit) (clone W18195C) (closed histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by goat anti-rat IgG PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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375302 | 100 µg | 141€ |
CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human CD117 transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone W18195C antibody does not block the binding of clones 104D2 or S18022G to the target. Clone W18195C antibody does not bind to the target after cells are fixed by 4% PFA.
- RRID
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AB_2890812 (BioLegend Cat. No. 375302)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
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Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- Stem cell factor (c-kit ligand)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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1. Giebel LB, et al. 1992. Oncogene. 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. - Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
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