- Clone
- AE-1/AE-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118 AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AE-1slashAE-3_PURE_Cytokeratin_Antibody_IHC-P_1_103118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-Pan-Cytokeratin antibody (clone AE-1/AE-3) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 1 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, AEC, Cat. No. 929401) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified Pan-Cytokeratin clone AE-1/AE-3, conjugated and detected with a Cy3 conjugated CODEX™ oligonucleotide duplex (red). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
914204 | 100 µg | 184€ |
AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.
Product DetailsProduct Details
- Reactivity
- Human,Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using human epidermal keratins.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
- RRID
-
AB_2616960 (BioLegend Cat. No. 914204)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- NA
- UniProt
- View information about Pan-Cytokeratin on UniProt.org
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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