Ultra-LEAF™ Purified anti-human CD14 Antibody

Product Details

Reactivity

Human,Cynomolgus,Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full-length human CD14 protein

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation⁴, and immunofluorescence microscopy⁵. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

Product Citations

1. Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed

RRID

AB_2814124 (BioLegend Cat. No. 301861)
AB_2814125 (BioLegend Cat. No. 301862)

Antigen Details

Structure

GPI-linked membrane glycoprotein, 53-55 kD

Distribution

Monocytes, macrophages, granulocytes (low)

Function

LPS receptor, clearance of Gram-negative pathogens

Ligand/Receptor

LPS

Cell Type

Granulocytes, Macrophages, Monocytes, Neutrophils

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience

Molecular Family

CD Molecules

Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID

929 View all products for this Gene ID

UniProt

View information about CD14 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis