Instructions for Use
BioLegend's Fluorescence Spectra Analyzer is useful for the analysis of excitation and emission spectra of commonly used fluorochromes for flow cytometry.

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PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.
1. View Spectra
Normalized emission curves are plotted into each channel off the violet (V), blue (B) and red (R) lasers of the Cytek™ Aurora and Northern Lights flow cytometers. To view spectra, simply select a fluorophore from one of the dropdowns and then check or uncheck the box to toggle on or off. The displayed emission spectrum color for each fluorophore is indicated to the right.
2. Zoom In, Zoom Out, and Pan
To help with pinpointing exact data, the analyzer has the capacity to zoom in, allowing you easily visualize spectra intersection points with laser lines, filter sets and other spectra. To zoom in, double click your mouse over the area that you wish to zoom, or roll the mouse wheel forward. To zoom out, click on the zoom out button at the top right corner or roll the mouse wheel backward. To pan, click anywhere within the graph and move the cursor to your desired position. To reset the data to the original layout, click on the Reset Zoom button.
3. Customize Colors
All the colors for spectra, laser lines, and filters are customizable. After you have made your selections, click the Customize Colors button to open the interface panel. To change colors, click on the desired color box. Select colors by dragging the locator within the box gradient and/or use the color slider. You can also enter in RGB values if known. To go back to the default color, click on the top right box which shows the default color.
4. Export Image
To export and save the data as an image, click the Export Spectra button. A new window with the data will appear as a PNG file. Save it by right clicking and select “Save image as” or select “Copy image” to paste into another image editor.
  Custom Color Panel
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