- Clone
- 2B8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- c-KIT, Stem Cell Factor Receptor (SCFR)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a-2B8_Alx488_020708 a-2B8_Alx488_020708](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2B8_Alx488_020708.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 488 -
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CD117 (clone 2B8) Alexa Fluor® 488 (green), and anti-mouse CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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105815 | 25 µg | 81€ | ||||
105816 | 100 µg | 184€ |
CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Application References
-
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_493473 (BioLegend Cat. No. 105815)
AB_493472 (BioLegend Cat. No. 105816)
Antigen Details
- Structure
- Ig superfamily, 145 kD
- Distribution
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Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5. - Gene ID
- 16590 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD117
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD117
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,IHC-F
- UniProt
- View information about CD117 on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD117 (c-Kit)
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Biotin anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with B220 APC a... -
FITC anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells were stained with CD45R/B220 PE an... -
PE anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC -
PE/Cyanine5 anti-mouse CD117 (c-Kit)
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Purified anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC -
PE/Cyanine7 anti-mouse CD117 (c-Kit)
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b... -
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Pacific Blue™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... -
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... -
PerCP anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... -
APC/Cyanine7 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)
Confocal image of C57BL/6 mouse small intestine sample acqui... Confocal image of human lymph node sample acquired using the... -
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready)
Mouse bone marrow cells stained with 164Dy anti-Sca-1 (D7) a... -
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Brilliant Violet 711™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Alexa Fluor® 594 anti-mouse CD117 (c-Kit)
C57BL/6 mouse frozen testis section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis secti... -
APC/Fire™ 750 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
Brilliant Violet 510™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b Alex... -
Brilliant Violet 785™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b Alex... -
TotalSeq™-A0012 anti-mouse CD117 (c-kit)
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Brilliant Violet 605™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Alexa Fluor® 700 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
TotalSeq™-B0012 anti-mouse CD117 (c-Kit)
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TotalSeq™-C0012 anti-mouse CD117 (c-Kit)
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Spark NIR™ 685 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Brilliant Violet 650™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™)
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