- Clone
- J099B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histidine Tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![aJ099B12_WB_042612 aJ099B12_WB_042612](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/J099B12_PURE_His-tag_Antibody_111717_UPDATED.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot analysis of cell lysates from HeLa cells transfected without or with His-tagged plasmid using His Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control. -
Immunofluorescence of HeLa cells transfected without (A) or with (B) His-tagged protein plasmid using anti-His Mouse primary Antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B) is 1/100. -
Insect cell supernatant expressing His-tagged mouse CCL22 was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-His Tag antibody (clone J099B12). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml (Dilution 1:1000 - 1:5000). For immunocytochemistry, a concentration range of 0.1 - 0.5 μg/ml (Dilution 1:1000 - 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
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- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Product Citations
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- RRID
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AB_11204080 (BioLegend Cat. No. 652501)
AB_11204427 (BioLegend Cat. No. 652502)
AB_2564271 (BioLegend Cat. No. 652505)
Antigen Details
- Function
- Recombinant poly-histidine tag detection
- Biology Area
- Cell Biology
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- His Tag
- Specificity Alt (DOES NOT SHOW ON TDS):
- His Tag
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ICC
- UniProt
- View information about His Tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All His Tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-His Tag | J099B12 | WB,ICC |
HRP anti-His Tag | J099B12 | WB,ELISA |
Ultra-LEAF™ Purified anti-His Tag | J099B12 | WB |
Alexa Fluor® 488 anti-His Tag | J099B12 | ICC |
Alexa Fluor® 594 anti-His Tag | J099B12 | ICC |
Alexa Fluor® 647 anti-His Tag | J099B12 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-His Tag
Western blot analysis of cell lysates from HeLa cells transf... Immunofluorescence of HeLa cells transfected without (A) or ... Insect cell supernatant expressing His-tagged mouse CCL22 wa... -
HRP anti-His Tag
Whole cell extracts (15 µg total protein) from HEK293 cells ... ELISA assay was performed by coating a 96-well plate with 10... -
Ultra-LEAF™ Purified anti-His Tag
0.1µl recombinant Posi-Tag Epitope Tag Protein was resolved ... -
Alexa Fluor® 488 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wi... -
Alexa Fluor® 594 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wit... -
Alexa Fluor® 647 anti-His Tag
Hela cells transfected with multi-tag plasmid were fixed wit...
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