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7-AAD Viability Staining Solution

Pricing & Availability
Regulatory Status
RUO
Other Names
Viability dye, dead cell exclusion dye, cell viability dye
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Product Citations
324 publications
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420403 200 tests 24€
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420404 500 tests 48€
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Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation
CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration
50 µg/ml
Storage & Handling
Protect from light. Store between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 

Excitation Laser
Blue Laser (488 nm)
Application Notes

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References
  1. Paterson AM, et al. 2011. J. Immunol. 187:1097. PubMed
  2. Wingren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000. PubMed
  3. Sandu SK, et al. 2012. PNAS 109:20047. PubMed
  4. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
  5. Choudhury KR, et al. 2012. PLoS One. 7:e51415. PubMed
  6. Wu Y, et al. 2013. J. Immunol. 190:1859. PubMed
  7. Harker KS, et al. 2013. J. Leukoc Biol. 93:789. PubMed
  8. Zhang ZN, et al. 2013. Clin Chem. 59:1175. PubMed
  9. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
  10. Cohen HB, et al. 2013. Blood. 122:1935. PubMed
  11. Hufbauer M, et al. 2013. J. Virol. 87:12158. PubMed
  12. Turner JE, et al. 2103. J Exp Med. PubMed
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Product Citations
  1. Dokun A, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 790-803. PubMed
  2. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  3. McCaw L, et al. 2017. EBioMedicine. 15:24-35. PubMed
  4. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  5. Kulpa DA, et al. 2019. J Virol. 93:e00969-19. PubMed
  6. Van Loenhout J, et al. 2019. Cancers (Basel). 1.567361111. PubMed
  7. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  8. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  9. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  10. An S, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  12. Manolakou T, et al. 2022. Sci Adv. 8:eabo5840. PubMed
See More

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Schmid I, et al. 1992. Cytometry. 13:204

Gene ID
NA

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

Go To Top Version: 2    Revision Date: 05-21-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


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