Biotinylated Recombinant Human FOLR1 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Adult folate-binding protein (FBP), Folate receptor 1, Folate receptor, adult KB cells FBP, Ovarian tumor-associated antigen MOv18
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Product Citations
publications
Human_FOLR1_1_CF
Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid-BSA conjugate) in a dose-dependent manner. The ED50 for this effect is 0.5-3.0 ng/mL.
  • Human_FOLR1_1_CF
    Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid-BSA conjugate) in a dose-dependent manner. The ED50 for this effect is 0.5-3.0 ng/mL.
  • Human_FOLR1_2_CF
    Stability Testing for Recombinant Human FOLR1. Biotinylated Recombinant Human FOLR1 was aliquoted in PBS, pH 7.0 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to FA-BSA (folic acid-BSA conjugate) in a dose-dependent manner. The ED50 for this effect is 0.5-3.0 ng/mL
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565504 25 µg 284€
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565506 100 µg 708€
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Description

FOLR1, also known as folate receptor alpha, is a glycosylphosphatidylinositol (GPI)-anchored cell-surface glycoprotein encoded by the FOLR1 gene. FOLR1 binds with high affinity to folic acid and its derivatives and mediates cellular processes, including cell division, proliferation, and tissue growth via signaling cascades and components of the folate cycle. Each FOLR only binds one folate and brings the folate into cells via endocytosis of the FOLR-folate complex. Human FOLR1 shares 83% amino acid sequence homology with mouse and rat FOLR1. FOLR1, the most extensively studied isoform, has limited tissue expression but is overexpressed in several tumors of epithelial origin including ovarian cancer, non-small cell lung adenocarcinoma, breast cancer, renal cancer and endometrial cancer. The overexpression of FOLR1 is often associated with increased cancer progression and poor patient prognosis. It plays a critical role during embryogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human FOLR1, amino acid (Arg25-Met233) (Accession: # P15328) with a C-terminal 8His and Avi tag, was expressed in CHO cells.
Molecular Mass
The 246 amino acid recombinant protein has a predicted molecular mass of approximately 28.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 35-45 kD and 35-40 kD respectively as determined by SDS-PAGE. The predicted N-terminal amino acid is Arg.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at - 20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid BSA conjugate). The EC50 of this effect is 0.5 – 3.0 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
GPI-anchored N-glycosylated
Distribution

Plasma membrane, GPI-anchored, ER membrane, endosome, limited expression on the apical surfaces of the epithelial cells of normal lung, breast, thyroid, parathyroid, and kidney tissues 

Function
Folate receptor (FOLR1) binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. It is required for embryonic development and cell proliferation.
Ligand/Receptor
Folate and folic acid derivatives
Bioactivity
Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid BSA conjugate). The EC50 of this effect is 0.5 – 3.0 ng/mL.
Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Carrier Proteins
Antigen References
  1. Luhrs CA & Slomiany BL. 1989. J Biol Chem. 264:21446-9.
  2. Sierra EEP & Goldman ID. 1999. Semin Oncol. 26:11-23.
  3. Cheung A, et al. 2018. Clin Cancer Res. 24:5098-111.
  4. Cheung A, et al. 2016. Oncotarget. 7:52553–74.
  5. Kelemen LE. 2006. Int J Cancer. 119:243-50.
  6. Spannuth WA, et al. 2010. Expert Opin Biol Ther. 10:431-437.
  7. Kelemen LE, et al. 2004. Cancer Causes Control. 15:1085-93.
  8. Kelemen LE, et al. 2008. Cancer Res. 68:2498-506.
  9. Liu JJ, et al. 2010. Adv Genet. 71:79-121.
  10. Kotsopoulos J, et al. 2010. Int J Cancer. 126:2191-2198.
  11. Nawab FN and Kipreos ET. 2022. Trends Endocrinol Metab. 33:159-174.
  12. Piedrahita JA, et al. 1999. Nat. Genet. 23:228.
Gene ID
2348 View all products for this Gene ID
UniProt
View information about FOLR1 on UniProt.org
Go To Top Version: 1    Revision Date: 08-18-2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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