- Clone
- 9E10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- myc tag, c-Myc tag, cMyc tag, Myc epitope tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9E10_DirectBlotHRP_cmyc_Antibody_WB_021716 9E10_DirectBlotHRP_cmyc_Antibody_WB_021716](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E10_DirectBlotHRP_cmyc_Antibody_WB_021716.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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626804 | 25 µL | 81€ | ||||
626803 | 100 µL | 231€ |
The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino acids 408-439, C-terminal region of human c-myc
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
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*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
- Application References
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- Rao H, et al. 2001. Nature. 410:955.
- Peters CS, et al. 2001. J Biol Chem. 276:13718.
- Munro S, Pelham HR. 1987. Cell. 48:899.
- Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
- Product Citations
-
- RRID
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AB_2783361 (BioLegend Cat. No. 626804)
AB_2572009 (BioLegend Cat. No. 626803)
Antigen Details
- Biology Area
- Cell Biology, Protein Purification, Protein Synthesis
- Antigen References
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- Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
- Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
- Battey J, et al. 1983. Cell 34:779.
- Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- UniProt
- View information about c-Myc on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All c-Myc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-c-Myc | 9E10 | WB,ICC,IP,IHC-P,Purification |
Anti-c-Myc Tag (9E10) Affinity Gel | 9E10 | Purification,IP |
Biotin anti-c-Myc | 9E10 | WB,ICC |
Direct-Blot™ HRP anti-c-Myc | 9E10 | WB |
Alexa Fluor® 594 anti-c-Myc | 9E10 | ICC |
Ultra-LEAF™ Purified anti-c-Myc | 9E10 | WB,ELISA,ICC,IP,IHC-P,Purification |
Alexa Fluor® 647 anti-c-Myc | 9E10 | ICC |
Alexa Fluor® 488 anti-c-Myc Antibody | 9E10 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-c-Myc
Total cell lysates (15 µg protein) from 293E cells transfect... Whole cell extracts (15 µg total protein) from HeLa cells mi... Whole cell extracts (250 µg total protein) prepared from HeL... ICC staining of purified anti-c-Myc antibody (clone 9E10) on... IHC staining of anti-c-Myc antibody (clone 9E10) on formalin... -
Anti-c-Myc Tag (9E10) Affinity Gel
Binding capacity of anti-c-Myc agarose for C-terminal c-Myc ... Stable activity of anti-c-Myc agarose over ten rounds of aff... -
Biotin anti-c-Myc
Western blot analysis of 1, 0.5, 0.1 µl (lanes 1, 2, 3 respe... Immunofluorescence of Myc-tagged plasmid-transfected A549 ce... -
Direct-Blot™ HRP anti-c-Myc
Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10... -
Alexa Fluor® 594 anti-c-Myc
HeLa cells transiently transfected with c-Myc tag fused prot... HeLa cells transiently transfected with c-Myc tag fused prot... -
Ultra-LEAF™ Purified anti-c-Myc
Total cell lysates (15 µg protein) from 293E cells transfect... -
Alexa Fluor® 647 anti-c-Myc
Untransfected (panel A) or multi-tag transfected (panel B) H... -
Alexa Fluor® 488 anti-c-Myc Antibody
Untransfected (panel A) or multi-tag transfected (panel B) H...
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