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FITC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
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Product Citations
publications
145-2C11_FITC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
  • 145-2C11_FITC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
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100305 50 µg 24€
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100306 500 µg 80€
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  2. Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed
  3. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  4. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  5. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  6. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  7. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  8. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  9. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  10. Wu Y, et al. 2023. Nat Cancer. 4:382. PubMed
  11. Brunner SM, et al. 2023. Int J Mol Sci. 24:. PubMed
  12. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  13. Stitzlein LM, et al. 2023. Front Neurol. 14:1112207. PubMed
  14. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  15. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  16. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  17. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  18. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  19. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  20. Yang L, et al. 2023. Acta Pharmacol Sin. :. PubMed
  21. Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed
  22. Smith LA, et al. 2023. Front Oncol. 12:1031174. PubMed
  23. Murakata Y, et al. 2023. Front Cardiovasc Med. 9:1075358. PubMed
  24. Jerome JR, et al. 2023. Int J Mol Sci. 24: . PubMed
  25. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  26. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  27. Schulteis R, et al. 2008. Blood. 112:4905. PubMed
  28. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  29. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  30. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  31. Fornari T, et al. 2015. PLoS One. 10: 0142688. PubMed
  32. Koning B, et al. 2006. Int Immunol. 1.403472222. PubMed
  33. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  34. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  35. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  36. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  37. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  38. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  39. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  40. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  41. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  42. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  43. Lou F, et al. 2020. STAR Protoc. 1:100115. PubMed
  44. Patnode M, et al. 2013. Glycobiology. 23:381. PubMed
  45. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  46. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  47. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  48. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  49. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  50. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  51. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  52. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  53. Uchida A, et al. 2021. J Inflamm Res. 14:3089. PubMed
  54. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  55. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  56. Yin Q, et al. 2015. PLoS One. 10: 0137808. PubMed
  57. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  58. Matsumura K, et al. 2016. J Immunol. 197: 3233 - 3244. PubMed
  59. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  60. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  61. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  62. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  63. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  64. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  65. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  66. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  67. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  68. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  69. He L, et al. 2019. iScience. 0.790972222. PubMed
  70. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  71. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  72. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  73. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  74. Tang CC, et al. 2021. eLife. 10:00. PubMed
  75. Gong Y, et al. 2020. Cell Metabolism. 33(1):51-64.e9. PubMed
  76. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  77. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  78. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  79. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  80. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  81. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  82. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  83. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  84. Yang H, et al. 2022. Nutrients. 14:. PubMed
  85. Kondratieva T, et al. 2020. PLoS One. 15:e0239668. PubMed
  86. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  87. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  88. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  89. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  90. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  91. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  92. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  93. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  94. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  95. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  96. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  97. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  98. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  99. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  100. Bian G, et al. 2020. British Journal of Pharmacology. 178(3):636-653. PubMed
  101. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  102. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  103. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  104. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  105. Xu M, et al. 2013. J Immunol. 190:5436. PubMed
  106. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  107. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  108. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  109. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  110. Labiano S, et al. 2017. Oncoimmunology. 6:e1283468. PubMed
  111. Engblom C, et al. 2017. Science. 358:6367. PubMed
  112. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  113. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  114. Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed
  115. Yue W, et al. 2019. Nat Commun. 10:2025. PubMed
  116. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  117. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  118. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  119. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  120. Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
  121. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  122. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  123. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  124. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  125. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  126. Lin JC, et al. 2019. Nat Commun. 10:1057. PubMed
  127. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  128. Sánchez-del-Campo L, et al. 2021. J Exp Clin Cancer Res. 40:117. PubMed
  129. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  130. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  131. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  132. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  133. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  134. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  135. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  136. Sheng J, et al. 2021. eLife. 10:00. PubMed
  137. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  138. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  139. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  140. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  141. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  142. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  143. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  144. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  145. Ma C, et al. 2021. Mol Metab. 47:101170. PubMed
  146. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  147. L M, et al. 2016. Brain. 139: 1939-1957. PubMed
  148. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  149. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  150. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  151. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  152. Kanaya T, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:273. PubMed
  153. Zhou X, et al. 2021. Nutr Metab (Lond). 18:64. PubMed
  154. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  155. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  156. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  157. Amatruda M, et al. 2022. Brain Commun. 4:fcac044. PubMed
  158. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_312670 (BioLegend Cat. No. 100305)
AB_312671 (BioLegend Cat. No. 100306)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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