- Clone
- 4F7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Beta-dystroglycan, Beta-DG, DAG1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![4F7_HRP_beta-Dystroglycan_Antibody_1_041118 4F7_HRP_beta-Dystroglycan_Antibody_1_041118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4F7_HRP_beta-Dystroglycan_Antibody_1_041118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of HRP anti-β-Dystroglycan antibody (clone 4F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.5 µg/mL of clone 4F7 or mouse IgG overnight at 4°C. HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. The antibody also detects a smaller band, slightly higher than the 25 kD molecular weight marker, which corresponds to a cleavage product of β-dystroglycan as previously reported. -
IHC staining of HRP anti-β-Dystroglycan antibody (clone 4F7) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of HRP anti-β-Dystroglycan antibody (clone 4F7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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849403 | 25 µg | 104€ |
Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and utrophin scaffolds axin to the cytoskeleton. β-dystroglycan has also been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
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- Sumita K, et al. 2007. J. Neurochem. 100(1):154. (IP, WB)
- RRID
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AB_2734626 (BioLegend Cat. No. 849403)
Antigen Details
- Structure
- Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
- Distribution
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Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS.
Cellular distribution: Nucleus, plasma membrane, and cytoskeleton.
- Function
- β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
- Interaction
- Interacts with dystrophin, utrophin and other cytoskeletal components such as axin.
- Ligand/Receptor
- Receptor for dystrophin and utrophin.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules
- Antigen References
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1. Murphy S and Ohlendieck K. 2015. Comput. Struct. Biotechnol. J. Nov 26;14:20-7.
2. Moore CJ and Winder SJ. 2012. Neuromuscul. Disord. Nov: 22(11): 959. - Gene ID
- 1605 View all products for this Gene ID
- UniProt
- View information about beta-Dystroglycan on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All beta-Dystroglycan Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-β-Dystroglycan | 4F7 | WB,IHC-P,IP |
HRP anti-β-Dystroglycan | 4F7 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-β-Dystroglycan
Western blot of purified anti-β-Dystroglycan antibody (clone... IHC staining of purified anti-β-Dystroglycan antibody (clone... IHC staining of purified anti-β-Dystroglycan antibody (clone... -
HRP anti-β-Dystroglycan
Western blot of HRP anti-β-Dystroglycan antibody (clone 4F7)... IHC staining of HRP anti-β-Dystroglycan antibody (clone 4F7)... IHC staining of HRP anti-β-Dystroglycan antibody (clone 4F7)...
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