LEGEND MAX™ Human IL-29 (IFN-λ1) ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-29, IL29, Interleukin-29, Interleukin 29, IFN-λ1, IFNL1, interferon lambda 1
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Product Citations
publications
HU_IL29_IFNl1_LEGENDMAX_121917
  • HU_IL29_IFNl1_LEGENDMAX_121917
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446307 1 Pre-coated Plate 370€
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Description

Interleukin-29 (IL-29, IFN-λ1) is a member of the type III interferon family of cytokines. It shares a high degree (~80%) of amino acid sequence homology with both IL-28A (IFN-λ2) and IL-28B (IFN-λ3). IL-29 is produced by human peripheral blood mononuclear and dendritic cells in response to viral infection, TLR stimulation, or other type I IFN agonists. IL-29 has been demonstrated to upregulate MHC I expression on target cells; in addition, IL-29 also mediates the antiviral immune response to several viruses including Hepatitis B (HBV), Hepatitis C (HCV), and Human Immunodeficiency Virus (HIV). Increased serum levels of IL-29 have also been reported in Hashimoto’s thyroiditis patients.

The BioLegend LEGEND MAX™ Human IL-29 (IFN-λ1) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a polyclonal goat anti-human IL-29 capture antibody. The detection antibody is a biotinylated polyclonal goat anti-human IL-29 antibody. This kit is specifically designed for the accurate quantitation of human IL-29 (IFN-λ1) from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-human IL-29 Pre-coated 96-well Strip Microplate
  • Human IL-29 Dectection Antibody
  • Human IL-29 Standard
  • Avidin-HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Product Citations
  1. Li WW, et al. 2023. PLoS Pathog. e1011742:19. PubMed
Sensitivity
2.4 pg/mL
Standard Range
7.8 - 500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
282618 View all products for this Gene ID
UniProt
View information about IL-29 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 12-21-2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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