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Purified anti-CD14 Antibody

Pricing & Availability
Clone
W23074F (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, P08571, Myeloid cell-specific leucine-rich glycoprotein, Lipopolysaccaride (LPS) receptor
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W23074F_PURE_CD14_IHC-P_070824
IHC staining of Purified anti-CD14 (clone W23074F) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-CD14 (clone W23074F) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The tissue was also co-stained with Purified anti-human CD21 (clone Bu32) (Cat. No. 354902) followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature (panel C). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panel D). Scale bar: 50 µm
  • W23074F_PURE_CD14_IHC-P_070824
    IHC staining of Purified anti-CD14 (clone W23074F) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-CD14 (clone W23074F) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The tissue was also co-stained with Purified anti-human CD21 (clone Bu32) (Cat. No. 354902) followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature (panel C). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panel D). Scale bar: 50 µm
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651951 25 µg 144€
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651952 100 µg 364€
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Description

CD14 is a glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. It plays an important role in the innate immune system, and is the first pattern recognition receptor identified. CD14 primarily binds to bacterial lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria. By binding to LPS, CD14 initiates a signaling cascade that leads to the clearance of pathogens.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant peptide containing the full-length extracellular domain of human CD14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone W23074F) in immunohistochemistry with formalin-fixed, paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with either Sodium Citrate (Cat. No. 928502), or Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). 

This antibody (clone W23074F) is not suitable for western blot, flow cytometry or immunocytochemistry.

Antigen Details

Structure
CD14 is a 375 amino acid protein with a predicted molecular weight of 40.1 kD. Glycosylated/modified CD14 has a predicted molecular weight of ~53 kD.
Distribution

Cell surface/plasma membrane

Function
Receptor
Interaction
TLR4, MD-2
Ligand/Receptor
Bacterial lipopolysaccharide (LPS)
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References
  1. Stocks S, et al. 1990. Biochem. J. 268:275.
  2. Wright S, et al. 1990. Science. 249:1434.
  3. Sharygin D. et al. 2023. Immunology. 169:260-270.
Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 1    Revision Date: 07-08-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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