Purified anti-mouse CD209a (DC-SIGN) Antibody (Previously Covance catalog# MMS-5224)

Pricing & Availability
Clone
MMD3 (See other available formats)
Regulatory Status
RUO
Other Names
CD209 antigen-like protein A, dendritic cell-specific ICAM-3-grabbing non-integrin, CIRE, CD209, CDSIGN
Previously
Covance Catalog# MMS-5224
Isotype
Mouse IgG2c, κ
Ave. Rating
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Product Citations
publications
MMD3_Purified_CD209a_Antibody_FC_081017_updated
CHO-DC-SIGN transfected (filled histogram) & CHO-K1 (dashed histogram) cell lines were stained with Purified anti-mouse CD209a (clone MMD3) antibody, followed by PE anti-mouse IgG2a antibody (clone RMG2a-62).
  • MMD3_Purified_CD209a_Antibody_FC_081017_updated
    CHO-DC-SIGN transfected (filled histogram) & CHO-K1 (dashed histogram) cell lines were stained with Purified anti-mouse CD209a (clone MMD3) antibody, followed by PE anti-mouse IgG2a antibody (clone RMG2a-62).
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833001 100 µL 220€
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Description

CD209a, is also known as dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and CIRE. Of the eight DC-SIGN-related receptors in mice, CD209a is considered the closest homolog to human DC-SIGN (CD209). Although both mouse and human counterparts are expressed on monocyte-derived dendritic cells, expression variation has been noted. Mouse CD209a is found on skin and splenic myeloid cells and is highly expressed on plasmacytoid dendritic cells, while human CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. It plays a critical role in capturing and internalizing pathogens such as Streptococcus pneumoniae.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a purified extracellular domain of mouse CD209a (DC-SIGN)-IgG1 Fc fusion protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

FC

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

The optimal working dilution should be determined for each specific assay condition.
FC: 1 μg/test

Application Notes

This antibody is effective in Flow Cytometry (FC).

FC Positive Control: Mouse splenocytes

Product Citations
  1. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
RRID
AB_2564962 (BioLegend Cat. No. 833001)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Gene ID
170786 View all products for this Gene ID
UniProt
View information about CD209a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07-09-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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