- Clone
- W22006E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyclin Dependent Kinase Inhibitor 1A, CAP20, CIP1, WAF1, SDI1, p21, p21 CIP1, CDKN1, p21 Cip1/Waf1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A.
W22006E_PURE_p21_WB_072924 A.
W22006E_PURE_p21_WB_072924](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W22006E_PURE_p21_WB_072924.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-p21 (clone W22006E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: molecular weight. -
IHC staining with Purified anti-p21 on formalin-fixed paraffin-embedded human colon tissue was performed following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). The tissue sections were incubated without (panel A) or with Purified anti- p21 (clone W22006E) (panels B (in 10X) and panel C (in 40X)) overnight at 4°C followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). Scale Bar: 50 µm -
Serum-starved A431 cells (negative control, top panels) and A549 cells (positive control, bottom panels) were fixed and permeabilized with 4% PFA and ice-cold methanol. They were then intracellularly stained with Purified anti-p21 (clone W22006E) (right panels) or Purified rat IgG2b, κ isotype control (Cat. No. 400602) (left panels). This was followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) (red). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (clone C-11) (Cat. No. 628608) (green). Scale bar 50 µm. -
Serum starved MCF-7 cells (positive control, filled histogram), or A431 cells (negative control, open histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-p21 (clone W22006E) or Purified rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for either cell line) (Cat. No. 400602) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). The cells were co-stained with DAPI (Cat. No. 422801) for cell cycle analysis. Histogram represent cells gated on G1/G0.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
645951 | 25 µg | 132€ | ||||
645952 | 100 µg | 392€ |
p21 (also known as CIP1 or CDKN1A) is a G1 cell cycle regulator that inhibits cyclin-cyclin dependent kinase 2 and/or cyclin-cyclin dependent kinase 4 complexes. Its expression is highly controlled by the tumor suppressor protein p53, thus, p21 is involved in controlling the G1 cell cycle arrest in response to different stress stimuli. p21 plays a regulatory role in S phase DNA replication by interacting with PCNA (proliferating cell nuclear antigen). Activation of cyclin-dependent kinase 2 has been observed after p21 is cleaved by CASP3-like caspases. Loss of function of p21 has been associated with bladder cancer, Multiple Endocrine Neoplasia Type I and lymph node metastasis. In addition, p21 can bind the HIV integrase mediating resistance to infection in hematopoietic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant protein fragment of p21
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC, ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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For use of this antibody (clone W22006E) in immunocytochemistry (ICC), we recommend fixation/permeabilization with either PFA/Triton X-100 or PFA/Methanol. This antibody is not suitable for use with methanol fixation/permeabilization.
Antigen Details
- Structure
- p21 is a 164 amino acid length protein with an approximate molecular weight of 18 kD.
- Distribution
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Nuclear localization; in some diseases, it is localized in the cytoplasm.
- Function
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Control of cell cycle progression
Inhibition of cyclin-dependent kinase activity
Inhibits DNA synthesis by competing with POLD3
Play a role in p53 inhibition of cell proliferation in response to DNA damage - Interaction
- Nrf2, BCCIP, CIZ1, CUL4A, CCNE1, CDK, DDB1, DTL, GADD45A, GADD45G, HDAC, PCNA, PIM1, TK1, TSG101
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
- Molecular Family
- Tumor Suppressors
- Antigen References
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- Sorteberg AL, et al. 2022. Front Oncol. 12:906194.
- Galanos P, et al. 2016. Cell Cycle. 23:3153-3154.
- Lai L, et al. 2020. Int J Mol Sci. 21:8504.
- Xiao BD, et al. 2020. World Stem Cells. 12:481.
- Gene ID
- 1026 View all products for this Gene ID
- UniProt
- View information about p21 on UniProt.org
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